In vitro restoration of T cell immune function in human immunodeficiency virus-positive persons: Effects of interleukin (IL)-12 and anti-IL-10

Alan L. Landay, Mario Clerici, Farah Hashcmi, Harold Kessler, Jay A. Berzofsky, Gene M. Shearer

Research output: Contribution to journalArticle

Abstract

Cells from human immunodeficiency virus (HIV)-positive patients were evaluated for their in vitro responsiveness to recall antigen, alloantigen, and phytohemagglutinin (PHA) following the in vitro addition of interleukin (IL)-12 or anti-IL-10. Three-color flow cytometric analysis of CD4 and CD8 subsets was done to determine whether specific in vivo alterations in cell surface markers are associated with in vitro function changes. The results demonstrated a hierarchical response pattern to recall antigens versus alloantigen versus PHA, and these in vitro responses were associated with the number and activation status of CD4 cells. The in vitro addition of IL-12 or anti-IL-10 could restore antigen responses (HIV envelope peptides or influenza) in patients with 200-500 CD4 cells/μL; however, in patients with

Original languageEnglish
Pages (from-to)1085-1091
Number of pages7
JournalJournal of Infectious Diseases
Volume173
Issue number5
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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