In vitro selection and characterization of mutants in TEM-1-producing Escherichia coli by ceftazidime and ceftibuten

M. Perilli, B. Segatore, M. Tavio, D. Setacci, G. Celenza, F. De Santis, C. Pellegrini, G. M. Rossolini, Gianfranco Amicosante

Research output: Contribution to journalArticlepeer-review

Abstract

The present work was undertaken to study the ability of ceftazidime and ceftibuten to select in vitro resistant mutants of Escherichia coli HB101 harboring blaTEM-1 β-lactamase gene. Minimum inhibitory concentrations (MICs) of ceftazidime and ceftibuten were increased by a factor of 32, overcoming in the case of ceftazidime the breakpoint for clinical resistance. Outer membrane protein analysis and PCR for blaTEM alleles revealed that ceftazidime and ceftibuten select for different resistance mechanisms. Ceftazidime created mutants that encode an extended-spectrum β-lactamase (TEM-12) and exhibit decreased expression of OmpF. Ceftibuten was unable to select for extended-spectrum β-lactamase expressing mutants but reduced the expression of two porins, OmpC and OmpF. The stability of ceftibuten to hydrolysis and the difference in the structure of these β-lactam antibiotics could be responsible for the selection of different mechanisms of resistance.

Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalJournal of Chemotherapy
Volume19
Issue number2
Publication statusPublished - Apr 2007

Keywords

  • Ceftazidime
  • Ceftibuten
  • TEM-1 mutant

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Microbiology (medical)

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