In vitro selection of human immunodeficiency virus type 1 resistant to 3′-azido-3′-deoxythymidine

Ferdinando Dianzani, Guido Antonelli, Ombretta Turriziani, Guanmu Dong, Maria R. Capobianchi, Elisabetta Riva

Research output: Contribution to journalArticle

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Abstract

3′-Azido-3′-deoxythymidine (AZT)-resistant human immunodeficiency virus type 1 (HIV-1) was obtained by growing HTLV-IIIB in C8166 cell cultures in the presence of inhibitory concentrations of AZT. The AZT-resistant HIV-1 was capable of replicating, as measured by infectious virus yield, and inducing cytopathic effect in the presence of AZT concentrations able to completely suppress the replication of parental HTLV-IIIB. Cloning of the AZT-resistant HIV-1 revealed that a number of different variants of HIV-1 with various degrees of sensitivity to AZT emerged during propagation of HTLV-IIIB in C8166 cells in the presence of the drug. PCR experiments performed on DNA extracted from C8166 cells infected with a resistant strain revealed that viral DNA was produced in the presence of inhibitory concentrations of AZT, while viral DNA in C8166 cells infected with the parental virus was drastically inhibited. Reverse transcriptase isolated from the AZT-resistant HIV-1 variant failed to show resistance to AZT 5′-triphosphate.

Original languageEnglish
Pages (from-to)39-52
Number of pages14
JournalAntiviral Research
Volume18
Issue number1
DOIs
Publication statusPublished - 1992

Fingerprint

Zidovudine
HIV-1
Viral DNA
Viruses
RNA-Directed DNA Polymerase
Organism Cloning
Cell Culture Techniques
In Vitro Techniques
Polymerase Chain Reaction
DNA
Pharmaceutical Preparations

Keywords

  • AZT
  • AZT-resistant HIV-1
  • C8166 cell
  • HTLV-III

ASJC Scopus subject areas

  • Virology
  • Pharmacology

Cite this

In vitro selection of human immunodeficiency virus type 1 resistant to 3′-azido-3′-deoxythymidine. / Dianzani, Ferdinando; Antonelli, Guido; Turriziani, Ombretta; Dong, Guanmu; Capobianchi, Maria R.; Riva, Elisabetta.

In: Antiviral Research, Vol. 18, No. 1, 1992, p. 39-52.

Research output: Contribution to journalArticle

Dianzani, Ferdinando ; Antonelli, Guido ; Turriziani, Ombretta ; Dong, Guanmu ; Capobianchi, Maria R. ; Riva, Elisabetta. / In vitro selection of human immunodeficiency virus type 1 resistant to 3′-azido-3′-deoxythymidine. In: Antiviral Research. 1992 ; Vol. 18, No. 1. pp. 39-52.
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