Abstract
The aim of this study was to evaluate the in vitro steroid sensitivity as a predictor of clinical response to glucocorticoids in childhood idiopathic nephrotic syndrome (INS). Seventy-four patients (median age 4.33, interquartile range [IQR] 2.82–7.23; 63.5% male) were enrolled in a prospective multicenter study: in vitro steroid inhibition of patients' peripheral blood mononuclear cell proliferation was evaluated by [methyl-3H] thymidine incorporation assay at disease onset (T0) and after 4 weeks (T4) of treatment. Steroid dependence was associated with increased in vitro sensitivity at T4 assessed both as drug concentration inducing 50% of inhibition (IC50; odds ratio [OR] = 0.48, 95% confidence interval [CI] = 0.24–0.85; P = 0.0094) and maximum inhibition at the highest drug concentration (Imax; OR = 1.13, 95% CI = 1.02–1.31; P = 0.017). IC50 > 4.4 nM and Imax < 92% at T4 were good predictors for optimal clinical response. These results suggest that this test may be useful for predicting the response to glucocorticoid therapy in pediatric INS.
| Original language | English |
|---|---|
| Pages (from-to) | 268-274 |
| Number of pages | 7 |
| Journal | Clinical Pharmacology and Therapeutics |
| Volume | 100 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2016 |
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ASJC Scopus subject areas
- Pharmacology
- Pharmacology (medical)
Cite this
In vitro sensitivity to methyl-prednisolone is associated with clinical response in pediatric idiopathic nephrotic syndrome. / Cuzzoni, Eva; De Iudicibus, Sara; Stocco, Gabriele; Favretto, Diego; Pelin, Marco; Messina, Giovanni; Ghio, Luciana; Monti, E.; Pasini, Andrea; Montini, Giovanni; Decorti, Giuliana.
In: Clinical Pharmacology and Therapeutics, Vol. 100, No. 3, 2016, p. 268-274.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro sensitivity to methyl-prednisolone is associated with clinical response in pediatric idiopathic nephrotic syndrome
AU - Cuzzoni, Eva
AU - De Iudicibus, Sara
AU - Stocco, Gabriele
AU - Favretto, Diego
AU - Pelin, Marco
AU - Messina, Giovanni
AU - Ghio, Luciana
AU - Monti, E.
AU - Pasini, Andrea
AU - Montini, Giovanni
AU - Decorti, Giuliana
PY - 2016
Y1 - 2016
N2 - The aim of this study was to evaluate the in vitro steroid sensitivity as a predictor of clinical response to glucocorticoids in childhood idiopathic nephrotic syndrome (INS). Seventy-four patients (median age 4.33, interquartile range [IQR] 2.82–7.23; 63.5% male) were enrolled in a prospective multicenter study: in vitro steroid inhibition of patients' peripheral blood mononuclear cell proliferation was evaluated by [methyl-3H] thymidine incorporation assay at disease onset (T0) and after 4 weeks (T4) of treatment. Steroid dependence was associated with increased in vitro sensitivity at T4 assessed both as drug concentration inducing 50% of inhibition (IC50; odds ratio [OR] = 0.48, 95% confidence interval [CI] = 0.24–0.85; P = 0.0094) and maximum inhibition at the highest drug concentration (Imax; OR = 1.13, 95% CI = 1.02–1.31; P = 0.017). IC50 > 4.4 nM and Imax < 92% at T4 were good predictors for optimal clinical response. These results suggest that this test may be useful for predicting the response to glucocorticoid therapy in pediatric INS.
AB - The aim of this study was to evaluate the in vitro steroid sensitivity as a predictor of clinical response to glucocorticoids in childhood idiopathic nephrotic syndrome (INS). Seventy-four patients (median age 4.33, interquartile range [IQR] 2.82–7.23; 63.5% male) were enrolled in a prospective multicenter study: in vitro steroid inhibition of patients' peripheral blood mononuclear cell proliferation was evaluated by [methyl-3H] thymidine incorporation assay at disease onset (T0) and after 4 weeks (T4) of treatment. Steroid dependence was associated with increased in vitro sensitivity at T4 assessed both as drug concentration inducing 50% of inhibition (IC50; odds ratio [OR] = 0.48, 95% confidence interval [CI] = 0.24–0.85; P = 0.0094) and maximum inhibition at the highest drug concentration (Imax; OR = 1.13, 95% CI = 1.02–1.31; P = 0.017). IC50 > 4.4 nM and Imax < 92% at T4 were good predictors for optimal clinical response. These results suggest that this test may be useful for predicting the response to glucocorticoid therapy in pediatric INS.
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UR - http://www.scopus.com/inward/citedby.url?scp=84988981860&partnerID=8YFLogxK
U2 - 10.1002/cpt.372
DO - 10.1002/cpt.372
M3 - Article
VL - 100
SP - 268
EP - 274
JO - Clinical Pharmacology and Therapeutics
JF - Clinical Pharmacology and Therapeutics
SN - 0009-9236
IS - 3
ER -