In vitro stimulation of human tonsillar subepithelial B cells: Requirement for interaction with activated T cells

Mariella Dono, Simona Zupo, Rosanna Massara, Silvano Ferrini, Andrea Melagrana, Nicholas Chiorazzi, Manlio Ferrarini

Research output: Contribution to journalArticlepeer-review

Abstract

Human tonsillar subepithelial B cells, which are a marginal zone-equivalent B cell subset, respond readily to T-independent type 2 antigens, but not to polyclonal B cell activators in vitro. In this study, subepithelial (SE) B cells were induced to proliferate and mature into plasma cells when co-cultured with activated T cells. The response of SE B cells was not observed when co-cultures were carried out in transwell chambers or in the presence of blocking anti-LFA-1 antibodies, demonstrating the need for a close T-B cell interaction. The presence of soluble CD40 also prevented the B cell response in vitro suggesting a pivotal role of CD40-CD40 ligand interactions. The data are discussed in terms of the T cell dependence of marginal zone (MZ) B cell response and the possible existence of various MZ B cell subsets.

Original languageEnglish
Pages (from-to)752-756
Number of pages5
JournalEuropean Journal of Immunology
Volume31
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • CD40-CD40 ligand interaction
  • SE B cell activation
  • T cell B-cell contact

ASJC Scopus subject areas

  • Immunology

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