In vitro study: Binding of99m Tc-DPD to synthetic amyloid fibrils

Federica E. Buroni, Marco G. Persico, Lorenzo Lodola, Monica Concardi, Carlo Aprile

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This paper is an report of the investigation of the in vitro binding of99m Tc-DPD for synthetic amyloid fibrils used for the diagnosis of cardiac amyloidosis (CA), as compared with the use of99m Tc-HMDP and 99mTc-PPI. It also includes an inquiry into the role played by Ca2+ ions and serum proteins on binding to amyloid like materials, as well as the saturability and specificity of DPD for fibrils versus amorphous precipitates (AP). In the work, synthetic insulin fibrils (SIF) and AP were characterized by Congo red staining and TEM imaging. An equal amount of three radiopharmaceuticals were then added to fibrils in Ca2+ (0-4.2 mmol/L) or human serum (HS) adjoined samples and radiopharmaceutical uptake was assessed. To test the saturability of amyloid binding sites, a displacement assays with cold DPD was performed, while adding 50-1500 nmol of99m Tc-DPD to SIF or AP, saturation binding tests were subsequently carried out for evaluating its specificity for amyloid. Herein, synthetic fibrils and AP showed conformational differences at TEM and polarized microscopy analysis. In our study,99m Tc-DPD fibrils uptake was seen to be the highest and increased with calcium ions concentration. What is more, serum proteins reduced the bound fraction to the amyloid deposits of about 15%, and the Kd values of 90 nM and 114 nM relative to SIF and AP, respectively, did not significantly differ. We saw that99m Tc-DPD is the best seeker for amyloid fibrils in cardiac amyloidosis, and that Ca2+ concentration positively influenced DPD fibrils binding. Furthermore, the radioactivity bound to the serum protein clear up the idea of nuclide exchanging dynamic balance between amyloid and circulating proteins. Moreover, non-labeled DPD did not exert a competition for99m Tc-DPD binding sites, and, finally, DPD cannot be defined a radiopharmaceutical specific for amyloid deposits.

Original languageEnglish
Pages (from-to)231-235
Number of pages5
JournalCurrent Issues in Pharmacy and Medical Sciences
Volume28
Issue number4
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

Amyloid
Radiopharmaceuticals
Precipitates
Blood Proteins
Amyloid Plaques
Amyloidosis
Insulin
Binding Sites
Ions
Amyloidogenic Proteins
Congo Red
Deposits
Protein Binding
Radioactivity
Microscopy
Transmission electron microscopy
In Vitro Techniques
Staining and Labeling
Calcium
Isotopes

Keywords

  • tc-diphosphonates
  • Amyloid synthetic fibrils.
  • Cardiac amyloidosis
  • Nuclear medicine imaging

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Medicine(all)
  • Pharmacology

Cite this

In vitro study : Binding of99m Tc-DPD to synthetic amyloid fibrils. / Buroni, Federica E.; Persico, Marco G.; Lodola, Lorenzo; Concardi, Monica; Aprile, Carlo.

In: Current Issues in Pharmacy and Medical Sciences, Vol. 28, No. 4, 01.12.2015, p. 231-235.

Research output: Contribution to journalArticle

Buroni, Federica E. ; Persico, Marco G. ; Lodola, Lorenzo ; Concardi, Monica ; Aprile, Carlo. / In vitro study : Binding of99m Tc-DPD to synthetic amyloid fibrils. In: Current Issues in Pharmacy and Medical Sciences. 2015 ; Vol. 28, No. 4. pp. 231-235.
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