In vitro susceptibility of different human T-cell subpopulations and resistance of large granular lymphocytes to HTLV-1 infection

B. Macchi, M. Popovic, P. Allavena, J. Ortaldo, P. Rossi, R. C. Gallo, E. Bonmassar

Research output: Contribution to journalArticle

Abstract

T4 subpopulation of T lymphocytes is the preferential target of infection with human T leukemia/lymphoma virus of subgroup I (HTLV-I). In this study we attempt to determine whether different T-cell subsets exhibit differences in susceptibility to virus infection. T cells from cord or peripheral blood were separated according to cell densities and T-cell surface markers by Percoll gradient and Sepharose anti-Fab immunoadsorbent affinity column (IAC), respectively. Separated T-cell subpopulations were infected with HTLV-I, by means of co-cultivation with irradiated virus producer cell lines (MT-2, TK). Percentages of HTLV-I-infected cells were assayed by immunofluorescence assay (IFA), using highly specific mouse monoclonal antibody (MAb) directed against HTLV-I p19 core protein. The results showed that different T-cell subpopulations separated either by Percoll or by IAC were susceptible to HTLV-I infection with the exception of large granular lymphocytes (LGL), which exhibit high cell-mediated natural cytotoxicity (CMNC). The susceptibility to HTLV-I infection of T cells with CMNC activity was further studied on established cell clones with LGL morphology. The results showed again that these cells were resistant to the virus infection. The present studies indicate that different T-cell subpopulations, irrespective of their size and of cell-surface markers, are susceptible to HTLV-I infection, with the exception of functionally mature LGL or of immortalized LGL clones.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalInternational Journal of Cancer
Volume40
Issue number1
DOIs
Publication statusPublished - 1987

Fingerprint

Deltaretrovirus Infections
Human T-lymphotropic virus 1
Lymphoma
Leukemia
Lymphocytes
Viruses
T-Lymphocytes
Immunosorbents
Virus Diseases
Infection
Clone Cells
In Vitro Techniques
T-Lymphocyte Subsets
Cell Size
Sepharose
Fluorescent Antibody Technique
Cell Count
Monoclonal Antibodies
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

In vitro susceptibility of different human T-cell subpopulations and resistance of large granular lymphocytes to HTLV-1 infection. / Macchi, B.; Popovic, M.; Allavena, P.; Ortaldo, J.; Rossi, P.; Gallo, R. C.; Bonmassar, E.

In: International Journal of Cancer, Vol. 40, No. 1, 1987, p. 1-6.

Research output: Contribution to journalArticle

@article{a593df174ebf47c491afd12f7d7e325e,
title = "In vitro susceptibility of different human T-cell subpopulations and resistance of large granular lymphocytes to HTLV-1 infection",
abstract = "T4 subpopulation of T lymphocytes is the preferential target of infection with human T leukemia/lymphoma virus of subgroup I (HTLV-I). In this study we attempt to determine whether different T-cell subsets exhibit differences in susceptibility to virus infection. T cells from cord or peripheral blood were separated according to cell densities and T-cell surface markers by Percoll gradient and Sepharose anti-Fab immunoadsorbent affinity column (IAC), respectively. Separated T-cell subpopulations were infected with HTLV-I, by means of co-cultivation with irradiated virus producer cell lines (MT-2, TK). Percentages of HTLV-I-infected cells were assayed by immunofluorescence assay (IFA), using highly specific mouse monoclonal antibody (MAb) directed against HTLV-I p19 core protein. The results showed that different T-cell subpopulations separated either by Percoll or by IAC were susceptible to HTLV-I infection with the exception of large granular lymphocytes (LGL), which exhibit high cell-mediated natural cytotoxicity (CMNC). The susceptibility to HTLV-I infection of T cells with CMNC activity was further studied on established cell clones with LGL morphology. The results showed again that these cells were resistant to the virus infection. The present studies indicate that different T-cell subpopulations, irrespective of their size and of cell-surface markers, are susceptible to HTLV-I infection, with the exception of functionally mature LGL or of immortalized LGL clones.",
author = "B. Macchi and M. Popovic and P. Allavena and J. Ortaldo and P. Rossi and Gallo, {R. C.} and E. Bonmassar",
year = "1987",
doi = "10.1002/ijc.2910400102",
language = "English",
volume = "40",
pages = "1--6",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - In vitro susceptibility of different human T-cell subpopulations and resistance of large granular lymphocytes to HTLV-1 infection

AU - Macchi, B.

AU - Popovic, M.

AU - Allavena, P.

AU - Ortaldo, J.

AU - Rossi, P.

AU - Gallo, R. C.

AU - Bonmassar, E.

PY - 1987

Y1 - 1987

N2 - T4 subpopulation of T lymphocytes is the preferential target of infection with human T leukemia/lymphoma virus of subgroup I (HTLV-I). In this study we attempt to determine whether different T-cell subsets exhibit differences in susceptibility to virus infection. T cells from cord or peripheral blood were separated according to cell densities and T-cell surface markers by Percoll gradient and Sepharose anti-Fab immunoadsorbent affinity column (IAC), respectively. Separated T-cell subpopulations were infected with HTLV-I, by means of co-cultivation with irradiated virus producer cell lines (MT-2, TK). Percentages of HTLV-I-infected cells were assayed by immunofluorescence assay (IFA), using highly specific mouse monoclonal antibody (MAb) directed against HTLV-I p19 core protein. The results showed that different T-cell subpopulations separated either by Percoll or by IAC were susceptible to HTLV-I infection with the exception of large granular lymphocytes (LGL), which exhibit high cell-mediated natural cytotoxicity (CMNC). The susceptibility to HTLV-I infection of T cells with CMNC activity was further studied on established cell clones with LGL morphology. The results showed again that these cells were resistant to the virus infection. The present studies indicate that different T-cell subpopulations, irrespective of their size and of cell-surface markers, are susceptible to HTLV-I infection, with the exception of functionally mature LGL or of immortalized LGL clones.

AB - T4 subpopulation of T lymphocytes is the preferential target of infection with human T leukemia/lymphoma virus of subgroup I (HTLV-I). In this study we attempt to determine whether different T-cell subsets exhibit differences in susceptibility to virus infection. T cells from cord or peripheral blood were separated according to cell densities and T-cell surface markers by Percoll gradient and Sepharose anti-Fab immunoadsorbent affinity column (IAC), respectively. Separated T-cell subpopulations were infected with HTLV-I, by means of co-cultivation with irradiated virus producer cell lines (MT-2, TK). Percentages of HTLV-I-infected cells were assayed by immunofluorescence assay (IFA), using highly specific mouse monoclonal antibody (MAb) directed against HTLV-I p19 core protein. The results showed that different T-cell subpopulations separated either by Percoll or by IAC were susceptible to HTLV-I infection with the exception of large granular lymphocytes (LGL), which exhibit high cell-mediated natural cytotoxicity (CMNC). The susceptibility to HTLV-I infection of T cells with CMNC activity was further studied on established cell clones with LGL morphology. The results showed again that these cells were resistant to the virus infection. The present studies indicate that different T-cell subpopulations, irrespective of their size and of cell-surface markers, are susceptible to HTLV-I infection, with the exception of functionally mature LGL or of immortalized LGL clones.

UR - http://www.scopus.com/inward/record.url?scp=0023219026&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023219026&partnerID=8YFLogxK

U2 - 10.1002/ijc.2910400102

DO - 10.1002/ijc.2910400102

M3 - Article

C2 - 2885278

AN - SCOPUS:0023219026

VL - 40

SP - 1

EP - 6

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 1

ER -