In vitro synthesis of IgM rheumatoid factor by lymphocytes from patients with essential mixed cryoglobulinemia

P. L. Meroni, W. Barcellini, A. Sinico, A. Fornasieri, C. Sguotti, F. Invernizzi, G. D'Amico, C. Zanussi

Research output: Contribution to journalArticlepeer-review

Abstract

Peripheral blood mononuclear cells (PBMC) from patients with Essential Mixed Cryogloblinemia (EMC) were studied for their ability to synthesize polyclonal IgM and rheumatoid factor (RF) IgM in vitro. Our results indicate: (a) that EMC-PBMC produce smaller amounts of polyclonal IgM but higher quantities of IgM-RF than normal PBMC after pokeweed mitogen (PWM) or Staphylococcus aureus activation, so that the IgM-RF to total IgM ratio is significantly greater in EMC than in normal cultures; (b) that enriched EMC-B lymphocytes display a significantly higher spontaneous synthesis of IgM-RF than normal B lymphocytes and (c) that the IgM-RF B cell clones are receptive to T cell regulation. Taken together these findings suggest an expansion of B cell clones committed to IgM RF production and the presence in peripheral blood of differentiated B lymphocytes capable of secreting IgM-RF in EMC.

Original languageEnglish
Pages (from-to)303-310
Number of pages8
JournalClinical and Experimental Immunology
Volume65
Issue number2
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Immunology

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