In vitro synthesis of the gene coding for the glycoprotein E1 of Sindbis virus.

A. Leone, P. Castagnola, A. Cerasuolo, R. Cancedda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ds cDNA of the 42S virionic RNA of Sindbis Virus has been synthesized and cloned in the plasmid pBR 322. Restriction map analysis and hybridization studies show that two clones cover the 3' end non coding region of the RNA, the whole membrane glycoprotein E1 and a peptide of MW 6,000 daltons. The use of these clones in experiments of gene expression in mammalian cell is discussed.

Original languageEnglish
Pages (from-to)123-130
Number of pages8
JournalMicrobiologica
Volume8
Issue number2
Publication statusPublished - Apr 1985

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Clone Cells
Sindbis Virus
Untranslated RNA
RNA Viruses
Membrane Glycoproteins
Genes
Plasmids
Complementary DNA
Gene Expression
Peptides
In Vitro Techniques
Sindbis virus glycoprotein E1

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

In vitro synthesis of the gene coding for the glycoprotein E1 of Sindbis virus. / Leone, A.; Castagnola, P.; Cerasuolo, A.; Cancedda, R.

In: Microbiologica, Vol. 8, No. 2, 04.1985, p. 123-130.

Research output: Contribution to journalArticle

Leone, A, Castagnola, P, Cerasuolo, A & Cancedda, R 1985, 'In vitro synthesis of the gene coding for the glycoprotein E1 of Sindbis virus.', Microbiologica, vol. 8, no. 2, pp. 123-130.
Leone, A. ; Castagnola, P. ; Cerasuolo, A. ; Cancedda, R. / In vitro synthesis of the gene coding for the glycoprotein E1 of Sindbis virus. In: Microbiologica. 1985 ; Vol. 8, No. 2. pp. 123-130.
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