Abstract
Cytotoxicity of cadmium-containing silica nanoparticles Cd-SiO 2NPs (0.05-100 μg/mL) versus SiO2NPs and CdCl 2 was evaluated by an in vitro test battery in A549 by assessing (i) mitochondrial function, (ii) membrane integrity/cell morphology, (iii) cell growth/proliferation, (iv) apoptotic pathway, (v) oxidative stress, after short- (24-48 h) and long-term (10 days) exposure. Both Cd-SiO2NPs and CdCl2 produced dose-dependent cytotoxic effects: (i) MTT-assay: similar cytotoxicity pattern was observed at both 24 and 48 h, with a more Cd-SiO2NPs pronounced effect than CdCl2. Cd-SiO 2NPs induced mortality (about 50%) at 1 g/mL, CdCl2 at 25 g/mL; (ii) calcein-AM/PI staining: decrease in cell viability, noticeable at 25 g/mL, enhanced markedly at 50 and 100 g/mL, after 24 h. Cd-SiO2NPs induced higher mortality than CdCl2 (25% versus 4%, resp., at 25 g/mL) with further exacerbation after 48h; (iii) clonogenic assay: exposure for longer period (10 days) compromised the A549 proliferative capacity at very low dose (0.05 g/mL); (iv) a progressive activation of caspase-3 immunolabelling was detected already at 1 g/mL; (v) GSH intracellular level was modified by all compounds. In summary, in vitro data demonstrated that both Cd-SiO 2NPs and CdCl2 affected all investigated endpoints, more markedly after Cd-SiO2NPs, while SiO2NPs influenced GSH only.
| Original language | English |
|---|---|
| Article number | 931785 |
| Journal | Journal of Toxicology |
| Volume | 2013 |
| DOIs | |
| Publication status | Published - 2013 |
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ASJC Scopus subject areas
- Toxicology
- Pharmacology
Cite this
In vitro toxicity evaluation of engineered cadmium-coated silica nanoparticles on human pulmonary cells. / De Simone, Uliana; Manzo, Luigi; Profumo, Antonella; Coccini, Teresa.
In: Journal of Toxicology, Vol. 2013, 931785, 2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro toxicity evaluation of engineered cadmium-coated silica nanoparticles on human pulmonary cells
AU - De Simone, Uliana
AU - Manzo, Luigi
AU - Profumo, Antonella
AU - Coccini, Teresa
PY - 2013
Y1 - 2013
N2 - Cytotoxicity of cadmium-containing silica nanoparticles Cd-SiO 2NPs (0.05-100 μg/mL) versus SiO2NPs and CdCl 2 was evaluated by an in vitro test battery in A549 by assessing (i) mitochondrial function, (ii) membrane integrity/cell morphology, (iii) cell growth/proliferation, (iv) apoptotic pathway, (v) oxidative stress, after short- (24-48 h) and long-term (10 days) exposure. Both Cd-SiO2NPs and CdCl2 produced dose-dependent cytotoxic effects: (i) MTT-assay: similar cytotoxicity pattern was observed at both 24 and 48 h, with a more Cd-SiO2NPs pronounced effect than CdCl2. Cd-SiO 2NPs induced mortality (about 50%) at 1 g/mL, CdCl2 at 25 g/mL; (ii) calcein-AM/PI staining: decrease in cell viability, noticeable at 25 g/mL, enhanced markedly at 50 and 100 g/mL, after 24 h. Cd-SiO2NPs induced higher mortality than CdCl2 (25% versus 4%, resp., at 25 g/mL) with further exacerbation after 48h; (iii) clonogenic assay: exposure for longer period (10 days) compromised the A549 proliferative capacity at very low dose (0.05 g/mL); (iv) a progressive activation of caspase-3 immunolabelling was detected already at 1 g/mL; (v) GSH intracellular level was modified by all compounds. In summary, in vitro data demonstrated that both Cd-SiO 2NPs and CdCl2 affected all investigated endpoints, more markedly after Cd-SiO2NPs, while SiO2NPs influenced GSH only.
AB - Cytotoxicity of cadmium-containing silica nanoparticles Cd-SiO 2NPs (0.05-100 μg/mL) versus SiO2NPs and CdCl 2 was evaluated by an in vitro test battery in A549 by assessing (i) mitochondrial function, (ii) membrane integrity/cell morphology, (iii) cell growth/proliferation, (iv) apoptotic pathway, (v) oxidative stress, after short- (24-48 h) and long-term (10 days) exposure. Both Cd-SiO2NPs and CdCl2 produced dose-dependent cytotoxic effects: (i) MTT-assay: similar cytotoxicity pattern was observed at both 24 and 48 h, with a more Cd-SiO2NPs pronounced effect than CdCl2. Cd-SiO 2NPs induced mortality (about 50%) at 1 g/mL, CdCl2 at 25 g/mL; (ii) calcein-AM/PI staining: decrease in cell viability, noticeable at 25 g/mL, enhanced markedly at 50 and 100 g/mL, after 24 h. Cd-SiO2NPs induced higher mortality than CdCl2 (25% versus 4%, resp., at 25 g/mL) with further exacerbation after 48h; (iii) clonogenic assay: exposure for longer period (10 days) compromised the A549 proliferative capacity at very low dose (0.05 g/mL); (iv) a progressive activation of caspase-3 immunolabelling was detected already at 1 g/mL; (v) GSH intracellular level was modified by all compounds. In summary, in vitro data demonstrated that both Cd-SiO 2NPs and CdCl2 affected all investigated endpoints, more markedly after Cd-SiO2NPs, while SiO2NPs influenced GSH only.
UR - http://www.scopus.com/inward/record.url?scp=84886680158&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886680158&partnerID=8YFLogxK
U2 - 10.1155/2013/931785
DO - 10.1155/2013/931785
M3 - Article
C2 - 24194755
AN - SCOPUS:84886680158
VL - 2013
JO - Journal of Toxicology
JF - Journal of Toxicology
SN - 1687-8191
M1 - 931785
ER -