In vitro transformation of BALB c 3T3 cells by 1,1,2,2-tetrachloroethane

Annamaria Colacci, Paolo Perocco, Monica Vaccari, Mario Mazzullo, Adriana Albini, Silvio Parodi, Maurizio Taningher, Sandro Grilli

Research output: Contribution to journalArticle

Abstract

1,1,2,2-Tetrachloroethane (1,1,2,2-TTCE) was shown to be capable of inducing in vitro transformation of BALB c 3T3 cells (clone A-31) either in the presence or in the absence of S9 activating system using an amplification-transformation (level-II) assay by reseeding confluent cells from each treatment and allowing additional rounds of cell replication. In the absence of metabolic activation, the highest assayed dose (1000 μg/ml), exerting the highest toxicity, was the only transforming dose. Lower doses of 1,1,2,2-TTCE were capable of transforming BALB c cells in the presence of S9 activating system, the dose of 500 μg/ml exerting the highest transforming activity. The number and size of transformed foci recognized in the level-II plates were a function of the number of cells reseeded in the amplification assay. Foci obtained in the presence of S9 activating systems were larger in size, more deeply basophilic, and exhibited denser multilayering of constituent cells than foci recognized in the absence of exogenous metabolic activation.

Original languageEnglish
Pages (from-to)786-792
Number of pages7
JournalJapanese Journal of Cancer Research
Volume81
Issue number8
Publication statusPublished - Aug 1990

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BALB 3T3 Cells
Clone Cells
Cell Count
In Vitro Techniques
1,1,2,2-tetrachloroethane

Keywords

  • 1,1,2,2-Tetrachloroethane
  • BALB c 3T3
  • Transformation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Colacci, A., Perocco, P., Vaccari, M., Mazzullo, M., Albini, A., Parodi, S., ... Grilli, S. (1990). In vitro transformation of BALB c 3T3 cells by 1,1,2,2-tetrachloroethane. Japanese Journal of Cancer Research, 81(8), 786-792.

In vitro transformation of BALB c 3T3 cells by 1,1,2,2-tetrachloroethane. / Colacci, Annamaria; Perocco, Paolo; Vaccari, Monica; Mazzullo, Mario; Albini, Adriana; Parodi, Silvio; Taningher, Maurizio; Grilli, Sandro.

In: Japanese Journal of Cancer Research, Vol. 81, No. 8, 08.1990, p. 786-792.

Research output: Contribution to journalArticle

Colacci, A, Perocco, P, Vaccari, M, Mazzullo, M, Albini, A, Parodi, S, Taningher, M & Grilli, S 1990, 'In vitro transformation of BALB c 3T3 cells by 1,1,2,2-tetrachloroethane', Japanese Journal of Cancer Research, vol. 81, no. 8, pp. 786-792.
Colacci A, Perocco P, Vaccari M, Mazzullo M, Albini A, Parodi S et al. In vitro transformation of BALB c 3T3 cells by 1,1,2,2-tetrachloroethane. Japanese Journal of Cancer Research. 1990 Aug;81(8):786-792.
Colacci, Annamaria ; Perocco, Paolo ; Vaccari, Monica ; Mazzullo, Mario ; Albini, Adriana ; Parodi, Silvio ; Taningher, Maurizio ; Grilli, Sandro. / In vitro transformation of BALB c 3T3 cells by 1,1,2,2-tetrachloroethane. In: Japanese Journal of Cancer Research. 1990 ; Vol. 81, No. 8. pp. 786-792.
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