TY - JOUR
T1 - In vitro translation of chicken type X collagen in the presence of pancreas microsomes
AU - Castagnola, P.
AU - Cancedda, F. Descalzi
AU - Cancedda, R.
PY - 1986/4/14
Y1 - 1986/4/14
N2 - Total RNA from epiphysis of 17-day-old chick embryo tibiae was used to direct protein synthesis in a wheat germ cell free system. The type X collagen chain, identified on the basis of its electrophoretic migration and of peptides obtained by S. aureus V8 protease digestion, was the major translation product. The newly synthesized chain included a signal sequence that was removed when dog pancreas membranes were added at the time of the protein synthesis.
AB - Total RNA from epiphysis of 17-day-old chick embryo tibiae was used to direct protein synthesis in a wheat germ cell free system. The type X collagen chain, identified on the basis of its electrophoretic migration and of peptides obtained by S. aureus V8 protease digestion, was the major translation product. The newly synthesized chain included a signal sequence that was removed when dog pancreas membranes were added at the time of the protein synthesis.
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U2 - 10.1016/0006-291X(86)90883-1
DO - 10.1016/0006-291X(86)90883-1
M3 - Article
C2 - 2423075
AN - SCOPUS:0022539322
VL - 136
SP - 109
EP - 115
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -