Total RNA from epiphysis of 17-day-old chick embryo tibiae was used to direct protein synthesis in a wheat germ cell free system. The type X collagen chain, identified on the basis of its electrophoretic migration and of peptides obtained by S. aureus V8 protease digestion, was the major translation product. The newly synthesized chain included a signal sequence that was removed when dog pancreas membranes were added at the time of the protein synthesis.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - Apr 14 1986|
ASJC Scopus subject areas
- Molecular Biology