In vitro translation of chicken type X collagen in the presence of pancreas microsomes

P. Castagnola, F. Descalzi Cancedda, R. Cancedda

Research output: Contribution to journalArticlepeer-review

Abstract

Total RNA from epiphysis of 17-day-old chick embryo tibiae was used to direct protein synthesis in a wheat germ cell free system. The type X collagen chain, identified on the basis of its electrophoretic migration and of peptides obtained by S. aureus V8 protease digestion, was the major translation product. The newly synthesized chain included a signal sequence that was removed when dog pancreas membranes were added at the time of the protein synthesis.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume136
Issue number1
DOIs
Publication statusPublished - Apr 14 1986

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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