In vitro type-1 and type-2 cytokine production in systemic lupus erythematosus: Lack of relationship with clinical disease activity

W. Barcellini, G. P. Rizzardi, M. O. Borghi, F. Nicoletti, C. Fain, N. Del Papa, P. L. Meroni

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: to investigate the relationship between disease activity and in vitro cytokine, soluble(s)CD23 and polyclonal and anti-DNA antibody production by PBMC from patients with active systemic lupus erythematosus (SLE). Methods: cytokines, sCD23 and immunoglobulins were estimated by ELISA in unstimulated and polyclonal mitogen-stimulated culture supernatants. Results: PHA-induced IL-2 and IFN-γ production were decreased, whereas spontaneous and PHA-induced IL-6 and IL-10 production were increased in cultures of SLE lymphocytes. Conversely, spontaneous and PHA-stimulated IL-4 and sCD23 production was comparable between patients and controls. Finally, we found an increase in in vitro spontaneous polyclonal and anti-DNA IgG secretion. Conclusions: We demonstrated an expanded type-2 cytokine profile with no correlation with parameters of disease activity.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalLupus
Volume5
Issue number2
Publication statusPublished - 1996

Keywords

  • Cytokines
  • Polyclonal B cell activation
  • sCD23
  • SLE

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Fingerprint Dive into the research topics of 'In vitro type-1 and type-2 cytokine production in systemic lupus erythematosus: Lack of relationship with clinical disease activity'. Together they form a unique fingerprint.

Cite this