Abstract
Two hours after a single intraperitoneal injection of dexamethasone (20 μg/Kg b.w.) into adult male rats, a typical ladder of DNA fragments was detectable upon separation on agarose gels of DNA from thymocytes. This became maximally evident at 4 hours. Accumulation of sulfated glycoprotein-2 (SGP-2) mRNA, whose rate of expression has been associated to the processes of programmed cell death, preceded the appearance of DNA degradation, starting to increase as early as 30 min after steroid injection, and maintained higher than controls until 8 hrs; a different time course was shown by changes in the levels of beta-actin mRNA. In the spleen, under the same conditions, the SGP-2 message also increased at 30 min, prior to DNA fragmentation, but decreased thereafter below the control value.
| Original language | English |
|---|---|
| Pages (from-to) | 810-815 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 175 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 29 1991 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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In vivo accumulation of sulfated glycoprotein 2 mRNA in rat thymocytes upon dexamethasone-induced cell death. / Bettuzzi, S.; Troiano, L.; Davalli, P.; Tropea, F.; Ingletti, M. C.; Grassilli, E.; Monti, D.; Corti, A.; Franceschi, C.
In: Biochemical and Biophysical Research Communications, Vol. 175, No. 3, 29.03.1991, p. 810-815.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vivo accumulation of sulfated glycoprotein 2 mRNA in rat thymocytes upon dexamethasone-induced cell death
AU - Bettuzzi, S.
AU - Troiano, L.
AU - Davalli, P.
AU - Tropea, F.
AU - Ingletti, M. C.
AU - Grassilli, E.
AU - Monti, D.
AU - Corti, A.
AU - Franceschi, C.
PY - 1991/3/29
Y1 - 1991/3/29
N2 - Two hours after a single intraperitoneal injection of dexamethasone (20 μg/Kg b.w.) into adult male rats, a typical ladder of DNA fragments was detectable upon separation on agarose gels of DNA from thymocytes. This became maximally evident at 4 hours. Accumulation of sulfated glycoprotein-2 (SGP-2) mRNA, whose rate of expression has been associated to the processes of programmed cell death, preceded the appearance of DNA degradation, starting to increase as early as 30 min after steroid injection, and maintained higher than controls until 8 hrs; a different time course was shown by changes in the levels of beta-actin mRNA. In the spleen, under the same conditions, the SGP-2 message also increased at 30 min, prior to DNA fragmentation, but decreased thereafter below the control value.
AB - Two hours after a single intraperitoneal injection of dexamethasone (20 μg/Kg b.w.) into adult male rats, a typical ladder of DNA fragments was detectable upon separation on agarose gels of DNA from thymocytes. This became maximally evident at 4 hours. Accumulation of sulfated glycoprotein-2 (SGP-2) mRNA, whose rate of expression has been associated to the processes of programmed cell death, preceded the appearance of DNA degradation, starting to increase as early as 30 min after steroid injection, and maintained higher than controls until 8 hrs; a different time course was shown by changes in the levels of beta-actin mRNA. In the spleen, under the same conditions, the SGP-2 message also increased at 30 min, prior to DNA fragmentation, but decreased thereafter below the control value.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025904519&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(91)91637-R
DO - 10.1016/0006-291X(91)91637-R
M3 - Article
C2 - 2025255
AN - SCOPUS:0025904519
VL - 175
SP - 810
EP - 815
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -