In vivo and in vitro imaging using a multimodal optical system

Carmen R. Gigliotti, Luisa Altabella, Federico Boschi, Massimo Crippa, Antonello E. Spinelli

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bioluminescence in vitro studies are usually performed with dedicated microscopes. In this work, we proposed a novel image recovery algorithm and a multimodal system prototype to perform bioluminescence microscopy. A super resolution regularization algorithm (SRRA) was developed and applied in a feasibility study using Geant4 Monte Carlo (MC) simulation. The method was also tested using in vitro cells acquisition performed with the prototype. The results obtained with MC simulations showed an improvement in the spatial localization resolution from 90 μm to 10 μm. The processing of the in vitro mesothelioma cells image shows shrink of the localization full width half maximum (FWHM) from 110 μm to 13 μm.

Original languageEnglish
Title of host publication2015 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781467398626
DOIs
Publication statusPublished - Oct 3 2016
Event2015 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2015 - San Diego, United States
Duration: Oct 31 2015Nov 7 2015

Other

Other2015 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2015
CountryUnited States
CitySan Diego
Period10/31/1511/7/15

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ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Radiology Nuclear Medicine and imaging
  • Instrumentation

Cite this

Gigliotti, C. R., Altabella, L., Boschi, F., Crippa, M., & Spinelli, A. E. (2016). In vivo and in vitro imaging using a multimodal optical system. In 2015 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2015 [7582161] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NSSMIC.2015.7582161