In vivo and in vitro inhibitory effect of α-interferon on megakaryocyte colony growth in essential thrombocythaemia

L. Gugliotta, G. P. Bagnara, L. Catani, L. Gaggioli, A. Guarini, G. Zauli, M. M. Belmonte, F. Lauria, S. Macchi, S. Tura

Research output: Contribution to journalArticlepeer-review

Abstract

Megakaryocyte (MK) colony growth of bone marrow mononuclear non-adherent cells was evaluated in 28 patients with essential thrombocythaemia (ET) and in 26 normal controls. The number of MK-colony forming units (CFU-MK per 3 x 105 plated cells) was similar in ET (68 ± 33) and in controls (63 ± 37), independently of bone marrow accessory cells. On the contrary, the size of the MK colonies was significantly (P6 IU/d s.c. for 11 ± 3 weeks, was capable of inducing a significant (P9/l). When added in vitro at time 0 to the culture dishes, α-IFN inhibited the CFU-MK growth of both normal and ET bone marrow samples, even at very low concentrations (1 and 10 IU/ml). This study demonstrates that α-IFN, both in vivo and in vitro, exerts an inhibitory effect on the growth of MK progenitors, which appears to correlate with the clinically documented anti-proliferative effect of this cytokine.

Original languageEnglish
Pages (from-to)177-181
Number of pages5
JournalBritish Journal of Haematology
Volume71
Issue number2
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Hematology

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