TY - JOUR
T1 - In vivo and in vitro interaction of trichloroethylene with macromolecules from various organs of rat and mouse
AU - Mazzullo, M.
AU - Bartoli, S.
AU - Bonora, B.
AU - Colacci, A.
AU - Lattanzi, G.
AU - Niero, A.
AU - Silingardi, P.
AU - Grilli, S.
PY - 1992
Y1 - 1992
N2 - Trichloroethylene was covalently bound in vivo to DNA, RNA and proteins of rat and mouse organs 22 hr after ip injection. The covalent binding index values of rat and mouse liver DNA classify trichloroethylene as a weak initiator. Labeling of RNA and proteins from various organs of both species was higher than that of DNA. In vitro, trichloroethylene was bioactivated by microsomal fractions dependent on cytochrome P450, mainly from liver of both species, to intermediate(s) capable of binding to exogenous DNA. No particular species-specific difference was evident except for mouse lung microsomes which were more efficient than rat lung microsomes. GSH- transferases capable of bioactivating P450-dependent were present in mouse lung microsomes and in liver microsomes of both species. These data, along those previously reported, provide sufficient evidence for a weak ability of TCY to interact covalently with DNA.
AB - Trichloroethylene was covalently bound in vivo to DNA, RNA and proteins of rat and mouse organs 22 hr after ip injection. The covalent binding index values of rat and mouse liver DNA classify trichloroethylene as a weak initiator. Labeling of RNA and proteins from various organs of both species was higher than that of DNA. In vitro, trichloroethylene was bioactivated by microsomal fractions dependent on cytochrome P450, mainly from liver of both species, to intermediate(s) capable of binding to exogenous DNA. No particular species-specific difference was evident except for mouse lung microsomes which were more efficient than rat lung microsomes. GSH- transferases capable of bioactivating P450-dependent were present in mouse lung microsomes and in liver microsomes of both species. These data, along those previously reported, provide sufficient evidence for a weak ability of TCY to interact covalently with DNA.
UR - http://www.scopus.com/inward/record.url?scp=0026695524&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026695524&partnerID=8YFLogxK
M3 - Article
C2 - 1376481
AN - SCOPUS:0026695524
VL - 76
SP - 192
EP - 208
JO - Research Communications in Molecular Pathology and Pharmacology
JF - Research Communications in Molecular Pathology and Pharmacology
SN - 0034-5164
IS - 2
ER -