In vivo angiogenic activity of neuroblastoma correlates with MYCN oncogene overexpression

Domenico Ribatti, Lizzia Raffaghello, Fabio Pastorino, Beatrice Nico, Chiara Brignole, Angelo Vacca, Mirco Ponzoni

Research output: Contribution to journalArticle

Abstract

Neuroblastoma (NB) is the most common malignant solid tumor in early childhood. Amplification of the MYCN oncogene is associated with a more malignant course of disease and poor outcome. The role that MYCN plays in the regulation of angiogenesis in NB remains unclear. To better elucidate this matter, fresh biopsy samples from 21 patients, 10 with MYCN-amplified tumors (defined as having > 10 copies of the oncogene) and 11 with nonamplified tumors, were tested for their angiogenic capacity using the chick embryo chorioallantoic membrane assay, a useful model for such investigation. Moreover, using the same experimental model, conditioned media obtained from 5 different human NB cell lines MYCN-amplified (HTLA-230, LAN-5 and GI-LI-N) or nonamplified (ACN and SH-SY5Y) and biopsy fragments obtained from xenografts derived from 4 NB cell lines (HTLA-230, GI-LI-N, ACN and SH-SY5Y) injected in nude mice were assayed for angiogenic potential. Our results clearly demonstrated that MYCN amplification parallels angiogenesis in NB. When fresh biopsy samples from patients, CM derived from NB cell lines and biopsy fragments derived from xenografts of the same cell lines injected in nude mice were tested, the response was univocal: the angiogenic response, evaluated both macroscopically and microscopically, was significantly higher in the MYCN-amplified specimens compared to the nonamplified ones.

Original languageEnglish
Pages (from-to)351-354
Number of pages4
JournalInternational Journal of Cancer
Volume102
Issue number4
DOIs
Publication statusPublished - Dec 1 2002

Fingerprint

Neuroblastoma
Oncogenes
Biopsy
Cell Line
Heterografts
Nude Mice
Local Area Networks
Chorioallantoic Membrane
Neoplasms
Chick Embryo
Conditioned Culture Medium
Theoretical Models

Keywords

  • Angiogenesis
  • Chorioallantoic membrane
  • MYCN
  • Neuroblastoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

In vivo angiogenic activity of neuroblastoma correlates with MYCN oncogene overexpression. / Ribatti, Domenico; Raffaghello, Lizzia; Pastorino, Fabio; Nico, Beatrice; Brignole, Chiara; Vacca, Angelo; Ponzoni, Mirco.

In: International Journal of Cancer, Vol. 102, No. 4, 01.12.2002, p. 351-354.

Research output: Contribution to journalArticle

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