In vivo behaviour of neuraminidase-treated rabbit erythrocytes and reticulocytes

Carlo L. Balduini; Giovanni Ricevuti; Maria C. Sosso; Edoardo Ascari; Augusta Brovelli; Cesare Balduini

doi:10.1159/000207879

In vivo behaviour of neuraminidase-treated rabbit erythrocytes and reticulocytes

Carlo L. Balduini, Giovanni Ricevuti, Maria C. Sosso, Edoardo Ascari, Augusta Brovelli, Cesare Balduini

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

51Cr rabbit erythrocytes were treated with different amounts of neuraminidase and reinjected into the animal. The survival curves after the removal of more than 50°/o membrane sialic acid show a characteristic behaviour: after a rapid decrease, blood radioactivity increases again reaching a maximum level 50-80 h after reinjection, then tends to decrease with a slope similar to that of control curves. Liver radioactivity determined before the rise of blood radioactivity is evidently higher than the value determined after radioactivity elevation. Similar results were obtained with phenylhydrazine-induced young erythrocytes.

Original language	English
Pages (from-to)	178-187
Number of pages	10
Journal	Acta Haematologica
Volume	57
Issue number	3
DOIs	https://doi.org/10.1159/000207879
Publication status	Published - 1977

Keywords

Erythrocyte life-span
Erythrocyte membrane
Neuraminidase
Rabbit erythrocytes
Sialic acid

ASJC Scopus subject areas

Medicine (miscellaneous)
Hematology

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title = "In vivo behaviour of neuraminidase-treated rabbit erythrocytes and reticulocytes",

abstract = "51Cr rabbit erythrocytes were treated with different amounts of neuraminidase and reinjected into the animal. The survival curves after the removal of more than 50°/o membrane sialic acid show a characteristic behaviour: after a rapid decrease, blood radioactivity increases again reaching a maximum level 50-80 h after reinjection, then tends to decrease with a slope similar to that of control curves. Liver radioactivity determined before the rise of blood radioactivity is evidently higher than the value determined after radioactivity elevation. Similar results were obtained with phenylhydrazine-induced young erythrocytes.",

keywords = "Erythrocyte life-span, Erythrocyte membrane, Neuraminidase, Rabbit erythrocytes, Sialic acid",

author = "Balduini, {Carlo L.} and Giovanni Ricevuti and Sosso, {Maria C.} and Edoardo Ascari and Augusta Brovelli and Cesare Balduini",

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T1 - In vivo behaviour of neuraminidase-treated rabbit erythrocytes and reticulocytes

AU - Balduini, Carlo L.

AU - Ricevuti, Giovanni

AU - Sosso, Maria C.

AU - Ascari, Edoardo

AU - Brovelli, Augusta

AU - Balduini, Cesare

PY - 1977

Y1 - 1977

N2 - 51Cr rabbit erythrocytes were treated with different amounts of neuraminidase and reinjected into the animal. The survival curves after the removal of more than 50°/o membrane sialic acid show a characteristic behaviour: after a rapid decrease, blood radioactivity increases again reaching a maximum level 50-80 h after reinjection, then tends to decrease with a slope similar to that of control curves. Liver radioactivity determined before the rise of blood radioactivity is evidently higher than the value determined after radioactivity elevation. Similar results were obtained with phenylhydrazine-induced young erythrocytes.

AB - 51Cr rabbit erythrocytes were treated with different amounts of neuraminidase and reinjected into the animal. The survival curves after the removal of more than 50°/o membrane sialic acid show a characteristic behaviour: after a rapid decrease, blood radioactivity increases again reaching a maximum level 50-80 h after reinjection, then tends to decrease with a slope similar to that of control curves. Liver radioactivity determined before the rise of blood radioactivity is evidently higher than the value determined after radioactivity elevation. Similar results were obtained with phenylhydrazine-induced young erythrocytes.

KW - Erythrocyte life-span

KW - Erythrocyte membrane

KW - Neuraminidase

KW - Rabbit erythrocytes

KW - Sialic acid

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