In Vivo Chronic Stimulation Unveils Autoreactive Potential of Wiskott-Aldrich Syndrome Protein-Deficient B Cells

Maria Carmina Castiello, Francesca Pala, Lucia Sereni, Elena Draghici, Donato Inverso, Aisha V Sauer, Francesca Schena, Elena Fontana, Enrico Radaelli, Paolo Uva, Karla E Cervantes-Luevano, Federica Benvenuti, Pietro L Poliani, Matteo Iannacone, Elisabetta Traggiai, Anna Villa, Marita Bosticardo

Research output: Contribution to journalArticle

Abstract

Wiskott-Aldrich syndrome (WAS) is a primary immunodeficiency caused by mutations in the gene encoding the hematopoietic-specific WAS protein (WASp). WAS is frequently associated with autoimmunity, indicating a critical role of WASp in maintenance of tolerance. The role of B cells in the induction of autoreactive immune responses in WAS has been investigated in several settings, but the mechanisms leading to the development of autoimmune manifestations have been difficult to evaluate in the mouse models of the disease that do not spontaneously develop autoimmunity. We performed an extensive characterization of Was-/- mice that provided evidence of the potential alteration in B cell selection, because of the presence of autoantibodies against double-stranded DNA, platelets, and tissue antigens. To uncover the mechanisms leading to the activation of the potentially autoreactive B cells in Was-/- mice, we performed in vivo chronic stimulations with toll-like receptors agonists (LPS and CpG) and apoptotic cells or infection with lymphocytic choriomeningitis virus. All treatments led to increased production of autoantibodies, increased proteinuria, and kidney tissue damage in Was-/- mice. These findings demonstrate that a lower clearance of pathogens and/or self-antigens and the resulting chronic inflammatory state could cause B cell tolerance breakdown leading to autoimmunity in WAS.

Original languageEnglish
Pages (from-to)490
JournalFrontiers in Immunology
Volume8
DOIs
Publication statusPublished - 2017

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Wiskott-Aldrich Syndrome Protein
Wiskott-Aldrich Syndrome
B-Lymphocytes
Autoimmunity
Autoantibodies
Lymphocytic choriomeningitis virus
Toll-Like Receptors
Autoantigens
Proteinuria
Blood Platelets
Maintenance
Kidney
Antigens
Mutation
IgA receptor
DNA
Infection
Genes
Proteins

Keywords

  • Journal Article

Cite this

Castiello, M. C., Pala, F., Sereni, L., Draghici, E., Inverso, D., Sauer, A. V., ... Bosticardo, M. (2017). In Vivo Chronic Stimulation Unveils Autoreactive Potential of Wiskott-Aldrich Syndrome Protein-Deficient B Cells. Frontiers in Immunology, 8, 490. https://doi.org/10.3389/fimmu.2017.00490

In Vivo Chronic Stimulation Unveils Autoreactive Potential of Wiskott-Aldrich Syndrome Protein-Deficient B Cells. / Castiello, Maria Carmina; Pala, Francesca; Sereni, Lucia; Draghici, Elena; Inverso, Donato; Sauer, Aisha V; Schena, Francesca; Fontana, Elena; Radaelli, Enrico; Uva, Paolo; Cervantes-Luevano, Karla E; Benvenuti, Federica; Poliani, Pietro L; Iannacone, Matteo; Traggiai, Elisabetta; Villa, Anna; Bosticardo, Marita.

In: Frontiers in Immunology, Vol. 8, 2017, p. 490.

Research output: Contribution to journalArticle

Castiello, MC, Pala, F, Sereni, L, Draghici, E, Inverso, D, Sauer, AV, Schena, F, Fontana, E, Radaelli, E, Uva, P, Cervantes-Luevano, KE, Benvenuti, F, Poliani, PL, Iannacone, M, Traggiai, E, Villa, A & Bosticardo, M 2017, 'In Vivo Chronic Stimulation Unveils Autoreactive Potential of Wiskott-Aldrich Syndrome Protein-Deficient B Cells', Frontiers in Immunology, vol. 8, pp. 490. https://doi.org/10.3389/fimmu.2017.00490
Castiello, Maria Carmina ; Pala, Francesca ; Sereni, Lucia ; Draghici, Elena ; Inverso, Donato ; Sauer, Aisha V ; Schena, Francesca ; Fontana, Elena ; Radaelli, Enrico ; Uva, Paolo ; Cervantes-Luevano, Karla E ; Benvenuti, Federica ; Poliani, Pietro L ; Iannacone, Matteo ; Traggiai, Elisabetta ; Villa, Anna ; Bosticardo, Marita. / In Vivo Chronic Stimulation Unveils Autoreactive Potential of Wiskott-Aldrich Syndrome Protein-Deficient B Cells. In: Frontiers in Immunology. 2017 ; Vol. 8. pp. 490.
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AU - Sereni, Lucia

AU - Draghici, Elena

AU - Inverso, Donato

AU - Sauer, Aisha V

AU - Schena, Francesca

AU - Fontana, Elena

AU - Radaelli, Enrico

AU - Uva, Paolo

AU - Cervantes-Luevano, Karla E

AU - Benvenuti, Federica

AU - Poliani, Pietro L

AU - Iannacone, Matteo

AU - Traggiai, Elisabetta

AU - Villa, Anna

AU - Bosticardo, Marita

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N2 - Wiskott-Aldrich syndrome (WAS) is a primary immunodeficiency caused by mutations in the gene encoding the hematopoietic-specific WAS protein (WASp). WAS is frequently associated with autoimmunity, indicating a critical role of WASp in maintenance of tolerance. The role of B cells in the induction of autoreactive immune responses in WAS has been investigated in several settings, but the mechanisms leading to the development of autoimmune manifestations have been difficult to evaluate in the mouse models of the disease that do not spontaneously develop autoimmunity. We performed an extensive characterization of Was-/- mice that provided evidence of the potential alteration in B cell selection, because of the presence of autoantibodies against double-stranded DNA, platelets, and tissue antigens. To uncover the mechanisms leading to the activation of the potentially autoreactive B cells in Was-/- mice, we performed in vivo chronic stimulations with toll-like receptors agonists (LPS and CpG) and apoptotic cells or infection with lymphocytic choriomeningitis virus. All treatments led to increased production of autoantibodies, increased proteinuria, and kidney tissue damage in Was-/- mice. These findings demonstrate that a lower clearance of pathogens and/or self-antigens and the resulting chronic inflammatory state could cause B cell tolerance breakdown leading to autoimmunity in WAS.

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