In vivo confocal microscopy of ocular surface squamous neoplasia

R. Parrozzani, D. Lazzarini, A. Dario, E. Midena

Research output: Contribution to journalArticlepeer-review


PurposeTo analyse in vivostructural and cellular features of ocular surface squamous neoplasia using clinical confocal microscopy.MethodsTen consecutive cases of untreated ocular surface squamous neoplasia were in vivoinvestigated using clinical confocal microscopy (ConfoScan4, Nidek Co. Ltd, Gamagori, Japan) with a × 40 surface non-contact objective lens. Confocal microscopy images were compared with cytologic samples obtained by scraping technique. ResultsConfocal microscopy examination revealed large areas of superficial cells debris and/or keratin debris accompanied by syncytial-like groupings, loss of the normal structure of the conjunctival epithelium andor of the corneal basal epithelium layer, papillomatous organization, large fibrovascular structures, and fine vessels perpendicular to the tumour surface. Sub-epithelial (pre-Bowman) space involvement was documented in four cases (50%). Irregular healthy tissue infiltration at the lateral edge of the lesion was documented in two cases (20%) whereas abrupt demarcation between neoplastic cells and normal epithelium was documented in eight cases (80%). In vivocyto-morphologic study using clinical confocal microscopy showed cellular anisocytosis, pleocytosis, and anisonucleosis, enlarged nuclei with high nuclear to cytoplasmic ratio, high reflective cytoplasm and indistinct cytoplasmic borders in all cases (100%).ConclusionCCM appears to be a promising and non-invasive method for in vivostructural and cellular analysis of OSSN.

Original languageEnglish
Pages (from-to)455-460
Number of pages6
JournalEye (London, England)
Issue number4
Publication statusPublished - Apr 2011


  • carcinoma
  • confocal microscopy
  • conjunctiva
  • cytology
  • in vivo
  • neoplasia

ASJC Scopus subject areas

  • Ophthalmology

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