In vivo DNA gene elecro-transfer: A systematic analysis of different electrical parameters

Immacolata Zampaglione, Mirko Arcuri, Manuela Cappelletti, Gennaro Ciliberto, Gemma Perretta, Alfredo Nicosia, Nicola La Monica, Elena Fattori

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Intramuscular plasmid injection followed by electroporation is an efficient method for gene therapy or vaccination. Several protocols have been described that give good transduction levels with several reporter genes. Methods: In this work we have explored the efficiency of gene delivery upon variation of the different electrical parameters such as pulse length frequency and voltage monitoring both on short- and long-term protein production. Results: Having defined the best performing parameters, we have designed a short electric treatment that gives good levels of plasmid-encoded protein in different species such as mice, rabbits and monkeys.

Original languageEnglish
Pages (from-to)1475-1481
Number of pages7
JournalJournal of Gene Medicine
Volume7
Issue number11
DOIs
Publication statusPublished - Nov 2005

Keywords

  • Electrical parameters
  • Electroporation
  • Muscle

ASJC Scopus subject areas

  • Genetics

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