In vivo effect of human granulocyte-macrophage colony-stimulating factor on megakaryocytopoiesis

Massimo Aglietta, Clara Monzeglio, Fiorella Sanavio, Franco Apra, Silvia Morelli, Alessandra Stacchini, Wanda Piacibello, Federico Bussolino, GianPaolo Bagnara, Giorgio Zauli, Angelika C. Stern, F. Gavosto

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Abstract

The effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on megakaryocytopoiesis and platelet production was investigated in patients with normal hematopoiesis. Three findings indicated that GM-CSF plays a role in megakaryocytopoiesis. During treatment with GM-CSF (recombinant mammalian, glycosylated; Sandoz/Schering-Plough, 5.5 μg protein/kg/d, subcutaneously for 3 days) the percentage of megakaryocyte progenitors (megakaryocyte colony forming unit [CFU-Mk]) in S phase (evaluated by the suicide technique with high 3H-Tdr doses) increased from 31% ± 16% to 88% ± 11%; and the maturation profile of megakaryocytes was modified, with a relative increase in more immature stage I-III forms. Moreover, by autoradiography (after incubation of marrow cells with 125I-labeled GM-CSF) specific GM-CSF receptors were detectable on megakaryocytes. Nevertheless, the proliferative stimulus induced on the progenitors was not accompanied by enhanced platelet production (by contrast with the marked granulomonocytosis). It may be suggested that other cytokines are involved in the regulation of the intermediate and terminal-stages of megakaryocytopoiesis in vivo and that their intervention is an essential prerequisite to turn the GM-CSF-induced proliferative stimulus into enhanced platelet production.

Original languageEnglish
Pages (from-to)1191-1194
Number of pages4
JournalBlood
Volume77
Issue number6
Publication statusPublished - Mar 15 1991

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ASJC Scopus subject areas

  • Hematology

Cite this

Aglietta, M., Monzeglio, C., Sanavio, F., Apra, F., Morelli, S., Stacchini, A., Piacibello, W., Bussolino, F., Bagnara, G., Zauli, G., Stern, A. C., & Gavosto, F. (1991). In vivo effect of human granulocyte-macrophage colony-stimulating factor on megakaryocytopoiesis. Blood, 77(6), 1191-1194.