In Vivo Effects of Thymustimulin on Hematopoiesis of Mice Treated with Cyclophosphamide

Enrico Balleari, Giulio Massa, Cristina Musselii, Silvana Timitilli, Alessandra Battaglia, Riccardo Ghio

Research output: Contribution to journalArticlepeer-review


This study reports on the in vivo effects of thymustimulin (TST), a thymic extract, on the hematopoiesis of mice treated with cyclophosphamide (CTX). Peripheral blood counts and both bone marrow pluripotent (spleen colonyforming units, CFU-S) and committed (granulocyte-macrophage colony-forming units, CFU-GM; erythroid burst-forming units, BFU-E) hematopoietic progenitor cells were assayed by in vitro methods. Administration of CTX alone was associated with severe hemotoxicity, which was followed by a gradual recovery of hematopoiesis. Hematotoxic effect of CTX was less pronounced when TST was administered in association with CTX. All the studied parameters were higher in TST + CTX-treated animals than those in CTX-treated animals, especially after Day 5 from the beginning of treatment for blood leukocytes, bone marrow cell counts, and packed red cell volume, and at Day 10 or 15 for CFU-S, CFU-GM, and BFU-E. These findings suggest that, in this experimental model, the simultaneous administration of TST reduces the myelosuppressive activity of CTX in vivo.

Original languageEnglish
Pages (from-to)363-367
Number of pages5
JournalClinical Immunology and Immunopathology
Issue number3
Publication statusPublished - Sep 1993

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Immunology and Allergy
  • Immunology


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