Abstract
Bone-implant integration represents a major requirement to grant implant stability and reduce the risk of implant loosening. This study investigates the effect of progenitor cells and strontium-enriched hydrogel on the osseointegration of titanium implants. To mimic implant-bone interaction, an ectopic model was developed grafting Trabecular Titanium™ (TT) implants into decellularized bone seeded with human bone marrow mesenchymal stem cells (hBMSCs). TT was loaded or not with strontium-enriched amidated carboxymethylcellulose (CMCA) hydrogel and/or hBMSCs. Constructs were implanted subcutaneously in athymic mice and osteodeposition was investigated with microcomputed tomography (micro-CT), scanning electron microscopy (SEM), and pull-out test at 4, 8, and 12 weeks. Fluorescence imaging was performed at 8 and 12 weeks, histology at 4 and 8 weeks. Micro-CT demonstrated the homogeneity of the engineered bone in all groups, supporting the reproducibility of the ectopic model. Fluorescence imaging, histology, SEM and pull-out mechanical testing showed superior tissue ingrowth in TT implants loaded with both strontium-enriched CMCA and hBMSCs. In our model, the synergic action of the bioactive hydrogel and hBMSCs increased both the bone deposition and TT integration. Thus, we suggest that using orthopedic prosthetic implant preloaded with strontium-enriched CMCA and seeded with BMSCs could represent a valid single-step surgical strategy to improve implant osseointegration.
| Original language | English |
|---|---|
| Pages (from-to) | 448-456 |
| Number of pages | 9 |
| Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
| Volume | 103 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2015 |
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Keywords
- Contract grant number: Rf-iog-2007-647233
- Ectopic model italian ministry of health
- Hydrogel
- Mesenchymal stem cells
- Osseointegration
- Strontium
- Titanium
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Medicine(all)
Cite this
In vivo evaluation of bone deposition in macroporous titanium implants loaded with mesenchymal stem cells and strontium-enriched hydrogel. / Lovati, Arianna B.; Lopa, Silvia; Ò, Giuseppe Tal; Previdi, Sara; Recordati, Camilla; Mercuri, David; Segatti, Francesco; Zagra, Luigi; Moretti, Matteo.
In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 103, No. 2, 01.02.2015, p. 448-456.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vivo evaluation of bone deposition in macroporous titanium implants loaded with mesenchymal stem cells and strontium-enriched hydrogel
AU - Lovati, Arianna B.
AU - Lopa, Silvia
AU - Ò, Giuseppe Tal
AU - Previdi, Sara
AU - Recordati, Camilla
AU - Mercuri, David
AU - Segatti, Francesco
AU - Zagra, Luigi
AU - Moretti, Matteo
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Bone-implant integration represents a major requirement to grant implant stability and reduce the risk of implant loosening. This study investigates the effect of progenitor cells and strontium-enriched hydrogel on the osseointegration of titanium implants. To mimic implant-bone interaction, an ectopic model was developed grafting Trabecular Titanium™ (TT) implants into decellularized bone seeded with human bone marrow mesenchymal stem cells (hBMSCs). TT was loaded or not with strontium-enriched amidated carboxymethylcellulose (CMCA) hydrogel and/or hBMSCs. Constructs were implanted subcutaneously in athymic mice and osteodeposition was investigated with microcomputed tomography (micro-CT), scanning electron microscopy (SEM), and pull-out test at 4, 8, and 12 weeks. Fluorescence imaging was performed at 8 and 12 weeks, histology at 4 and 8 weeks. Micro-CT demonstrated the homogeneity of the engineered bone in all groups, supporting the reproducibility of the ectopic model. Fluorescence imaging, histology, SEM and pull-out mechanical testing showed superior tissue ingrowth in TT implants loaded with both strontium-enriched CMCA and hBMSCs. In our model, the synergic action of the bioactive hydrogel and hBMSCs increased both the bone deposition and TT integration. Thus, we suggest that using orthopedic prosthetic implant preloaded with strontium-enriched CMCA and seeded with BMSCs could represent a valid single-step surgical strategy to improve implant osseointegration.
AB - Bone-implant integration represents a major requirement to grant implant stability and reduce the risk of implant loosening. This study investigates the effect of progenitor cells and strontium-enriched hydrogel on the osseointegration of titanium implants. To mimic implant-bone interaction, an ectopic model was developed grafting Trabecular Titanium™ (TT) implants into decellularized bone seeded with human bone marrow mesenchymal stem cells (hBMSCs). TT was loaded or not with strontium-enriched amidated carboxymethylcellulose (CMCA) hydrogel and/or hBMSCs. Constructs were implanted subcutaneously in athymic mice and osteodeposition was investigated with microcomputed tomography (micro-CT), scanning electron microscopy (SEM), and pull-out test at 4, 8, and 12 weeks. Fluorescence imaging was performed at 8 and 12 weeks, histology at 4 and 8 weeks. Micro-CT demonstrated the homogeneity of the engineered bone in all groups, supporting the reproducibility of the ectopic model. Fluorescence imaging, histology, SEM and pull-out mechanical testing showed superior tissue ingrowth in TT implants loaded with both strontium-enriched CMCA and hBMSCs. In our model, the synergic action of the bioactive hydrogel and hBMSCs increased both the bone deposition and TT integration. Thus, we suggest that using orthopedic prosthetic implant preloaded with strontium-enriched CMCA and seeded with BMSCs could represent a valid single-step surgical strategy to improve implant osseointegration.
KW - Contract grant number: Rf-iog-2007-647233
KW - Ectopic model italian ministry of health
KW - Hydrogel
KW - Mesenchymal stem cells
KW - Osseointegration
KW - Strontium
KW - Titanium
UR - http://www.scopus.com/inward/record.url?scp=84920817189&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84920817189&partnerID=8YFLogxK
U2 - 10.1002/jbm.b.33228
DO - 10.1002/jbm.b.33228
M3 - Article
C2 - 24910213
AN - SCOPUS:84920817189
VL - 103
SP - 448
EP - 456
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 2
ER -