In vivo evaluation of bone deposition in macroporous titanium implants loaded with mesenchymal stem cells and strontium-enriched hydrogel

Arianna B. Lovati, Silvia Lopa, Giuseppe Tal Ò, Sara Previdi, Camilla Recordati, David Mercuri, Francesco Segatti, Luigi Zagra, Matteo Moretti

Research output: Contribution to journalArticlepeer-review

Abstract

Bone-implant integration represents a major requirement to grant implant stability and reduce the risk of implant loosening. This study investigates the effect of progenitor cells and strontium-enriched hydrogel on the osseointegration of titanium implants. To mimic implant-bone interaction, an ectopic model was developed grafting Trabecular Titanium™ (TT) implants into decellularized bone seeded with human bone marrow mesenchymal stem cells (hBMSCs). TT was loaded or not with strontium-enriched amidated carboxymethylcellulose (CMCA) hydrogel and/or hBMSCs. Constructs were implanted subcutaneously in athymic mice and osteodeposition was investigated with microcomputed tomography (micro-CT), scanning electron microscopy (SEM), and pull-out test at 4, 8, and 12 weeks. Fluorescence imaging was performed at 8 and 12 weeks, histology at 4 and 8 weeks. Micro-CT demonstrated the homogeneity of the engineered bone in all groups, supporting the reproducibility of the ectopic model. Fluorescence imaging, histology, SEM and pull-out mechanical testing showed superior tissue ingrowth in TT implants loaded with both strontium-enriched CMCA and hBMSCs. In our model, the synergic action of the bioactive hydrogel and hBMSCs increased both the bone deposition and TT integration. Thus, we suggest that using orthopedic prosthetic implant preloaded with strontium-enriched CMCA and seeded with BMSCs could represent a valid single-step surgical strategy to improve implant osseointegration.

Original languageEnglish
Pages (from-to)448-456
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume103
Issue number2
DOIs
Publication statusPublished - Feb 1 2015

Keywords

  • Contract grant number: Rf-iog-2007-647233
  • Ectopic model italian ministry of health
  • Hydrogel
  • Mesenchymal stem cells
  • Osseointegration
  • Strontium
  • Titanium

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

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