In vivo fate of avidin-nucleic acid nanoassemblies as multifunctional diagnostic tools

Paolo Bigini, Sara Previdi, Elisabetta Casarin, Davide Silvestri, Martina Bruna Violatto, Sonia Facchin, Leopoldo Sitia, Antonio Rosato, Gaia Zuccolotto, Nicola Realdon, Fabio Fiordaliso, Mario Salmona, Margherita Morpurgo

Research output: Contribution to journalArticlepeer-review

Abstract

This study describes the formulation optimization and body-cell distribution and clearance in mice of a dually fluorescent biodegradable poly avidin nanoassembly based on the novel Avidin-Nucleic-Acid-Nano-ASsembly (ANANAS) platform as a potential advancement of classic avidin/biotin-based targeted delivery. The nanoformulation circulates freely in the bloodstream; it is slowly captured by filter organs; it is efficiently cleared within 24-48 h, and it is poorly immunogenic. The system displays more favorable properties than its parent monomeric avidin and it is a promising tool for diagnostic purposes for future translational aims, for which free circulation in the bloodstream, safety, multifunctionality and high composition definition are all necessary requirements. In addition, the assembly shows a time-dependent cell penetration capability, suggesting it may also function as a NP-dependent drug delivery tool. The ease of preparation together with the possibility to fine-tune the surface composition makes it also an ideal candidate to understand if and how nanoparticle composition affects its localization.

Original languageEnglish
Pages (from-to)175-187
Number of pages13
JournalACS Nano
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 28 2014

Keywords

  • avidin nucleic acid nanoassemblies
  • fluorescence optical imaging
  • in vivo diagnostics
  • multifunctional
  • nanomedicine
  • poly avidin

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

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