Abstract
Objectives: To demonstrate that manganese can visualise calcium sensing receptor (CaSR)-expressing cells in a human breast cancer murine model, as assessed by clinical 3T magnetic resonance (MR). Methods: Human MDA-MB-231-Luc or MCF7-Luc breast cancer cells were orthotopically grown in NOD/SCID mice to a minimum mass of 5 mm. Mice were evaluated on T1-weighted sequences before and after intravenous injection of MnCl 2. To block the CaSR-activated Ca 2+ channels, verapamil was injected at the tumour site 5 min before Mn 2+ administration. CaSR expression in vivo was studied by immunohistochemistry. Results: Contrast enhancement was observed at the tumour periphery 10 min after Mn 2+ administration, and further increased up to 40 min. In verapamil-treated mice, no contrast enhancement was observed. CaSR was strongly expressed at the tumour periphery. Conclusion: Manganese enhanced magnetic resonance imaging can visualise CaSR-expressing breast cancer cells in vivo, opening up possibilities for a new MR contrast agent. Key Points : • Manganese contrast agents helped demonstrate breast cancer cells in an animal model. • Enhancement was most marked in cells with high calcium sensing receptor expression. • Manganese uptake was related to the distribution of CaSR within the tumour. • Manganese MRI may become useful to investigate human breast cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 551-558 |
| Number of pages | 8 |
| Journal | European Radiology |
| Volume | 22 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2012 |
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Keywords
- Breast cancer
- Calcium receptor
- In vivo small animal MRI
- Magnetic Resonance Imaging
- Manganese chloride
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
Cite this
In vivo imaging of human breast cancer mouse model with high level expression of calcium sensing receptor at 3T. / Baio, Gabriella; Fabbi, Marina; Emionite, Laura; Cilli, Michele; Salvi, Sandra; Ghedin, Piero; Prato, Sabina; Carbotti, Grazia; Tagliafico, Alberto; Truini, Mauro; Neumaier, Carlo Emanuele.
In: European Radiology, Vol. 22, No. 3, 03.2012, p. 551-558.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vivo imaging of human breast cancer mouse model with high level expression of calcium sensing receptor at 3T
AU - Baio, Gabriella
AU - Fabbi, Marina
AU - Emionite, Laura
AU - Cilli, Michele
AU - Salvi, Sandra
AU - Ghedin, Piero
AU - Prato, Sabina
AU - Carbotti, Grazia
AU - Tagliafico, Alberto
AU - Truini, Mauro
AU - Neumaier, Carlo Emanuele
PY - 2012/3
Y1 - 2012/3
N2 - Objectives: To demonstrate that manganese can visualise calcium sensing receptor (CaSR)-expressing cells in a human breast cancer murine model, as assessed by clinical 3T magnetic resonance (MR). Methods: Human MDA-MB-231-Luc or MCF7-Luc breast cancer cells were orthotopically grown in NOD/SCID mice to a minimum mass of 5 mm. Mice were evaluated on T1-weighted sequences before and after intravenous injection of MnCl 2. To block the CaSR-activated Ca 2+ channels, verapamil was injected at the tumour site 5 min before Mn 2+ administration. CaSR expression in vivo was studied by immunohistochemistry. Results: Contrast enhancement was observed at the tumour periphery 10 min after Mn 2+ administration, and further increased up to 40 min. In verapamil-treated mice, no contrast enhancement was observed. CaSR was strongly expressed at the tumour periphery. Conclusion: Manganese enhanced magnetic resonance imaging can visualise CaSR-expressing breast cancer cells in vivo, opening up possibilities for a new MR contrast agent. Key Points : • Manganese contrast agents helped demonstrate breast cancer cells in an animal model. • Enhancement was most marked in cells with high calcium sensing receptor expression. • Manganese uptake was related to the distribution of CaSR within the tumour. • Manganese MRI may become useful to investigate human breast cancer.
AB - Objectives: To demonstrate that manganese can visualise calcium sensing receptor (CaSR)-expressing cells in a human breast cancer murine model, as assessed by clinical 3T magnetic resonance (MR). Methods: Human MDA-MB-231-Luc or MCF7-Luc breast cancer cells were orthotopically grown in NOD/SCID mice to a minimum mass of 5 mm. Mice were evaluated on T1-weighted sequences before and after intravenous injection of MnCl 2. To block the CaSR-activated Ca 2+ channels, verapamil was injected at the tumour site 5 min before Mn 2+ administration. CaSR expression in vivo was studied by immunohistochemistry. Results: Contrast enhancement was observed at the tumour periphery 10 min after Mn 2+ administration, and further increased up to 40 min. In verapamil-treated mice, no contrast enhancement was observed. CaSR was strongly expressed at the tumour periphery. Conclusion: Manganese enhanced magnetic resonance imaging can visualise CaSR-expressing breast cancer cells in vivo, opening up possibilities for a new MR contrast agent. Key Points : • Manganese contrast agents helped demonstrate breast cancer cells in an animal model. • Enhancement was most marked in cells with high calcium sensing receptor expression. • Manganese uptake was related to the distribution of CaSR within the tumour. • Manganese MRI may become useful to investigate human breast cancer.
KW - Breast cancer
KW - Calcium receptor
KW - In vivo small animal MRI
KW - Magnetic Resonance Imaging
KW - Manganese chloride
UR - http://www.scopus.com/inward/record.url?scp=84856612236&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856612236&partnerID=8YFLogxK
U2 - 10.1007/s00330-011-2285-1
DO - 10.1007/s00330-011-2285-1
M3 - Article
C2 - 21947485
AN - SCOPUS:84856612236
VL - 22
SP - 551
EP - 558
JO - European Radiology
JF - European Radiology
SN - 0938-7994
IS - 3
ER -