Abstract
Objectives: To demonstrate that manganese can visualise calcium sensing receptor (CaSR)-expressing cells in a human breast cancer murine model, as assessed by clinical 3T magnetic resonance (MR). Methods: Human MDA-MB-231-Luc or MCF7-Luc breast cancer cells were orthotopically grown in NOD/SCID mice to a minimum mass of 5 mm. Mice were evaluated on T1-weighted sequences before and after intravenous injection of MnCl 2. To block the CaSR-activated Ca 2+ channels, verapamil was injected at the tumour site 5 min before Mn 2+ administration. CaSR expression in vivo was studied by immunohistochemistry. Results: Contrast enhancement was observed at the tumour periphery 10 min after Mn 2+ administration, and further increased up to 40 min. In verapamil-treated mice, no contrast enhancement was observed. CaSR was strongly expressed at the tumour periphery. Conclusion: Manganese enhanced magnetic resonance imaging can visualise CaSR-expressing breast cancer cells in vivo, opening up possibilities for a new MR contrast agent. Key Points : • Manganese contrast agents helped demonstrate breast cancer cells in an animal model. • Enhancement was most marked in cells with high calcium sensing receptor expression. • Manganese uptake was related to the distribution of CaSR within the tumour. • Manganese MRI may become useful to investigate human breast cancer.
Original language | English |
---|---|
Pages (from-to) | 551-558 |
Number of pages | 8 |
Journal | European Radiology |
Volume | 22 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2012 |
Keywords
- Breast cancer
- Calcium receptor
- In vivo small animal MRI
- Magnetic Resonance Imaging
- Manganese chloride
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging