In vivo imaging of lymph node migration of MNP- and 111In-labeled dendritic cells in a transgenic mouse model of breast cancer (MMTV-RAS)

Cristina Martelli, Manuela Borelli, Luisa Ottobrini, Veronica Rainone, Anna Degrassi, Micaela Russo, Umberto Gianelli, Silvano Bosari, Carlo Fiorini, Daria Trabattoni, Mario Clerici, Giovanni Lucignani

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: The authors present a protocol for the in vivo evaluation, using different imaging techniques, of lymph node (LN) homing of tumor-specific dendritic cells (DCs) in a murine breast cancer model. Procedures: Bone marrow DCs were labeled with paramagnetic nanoparticles (MNPs) or 111In- oxine. Antigen loading was performed using tumor lysate. Mature DCs were injected into the footpads of transgenic tumor-bearing mice (MMTV-Ras) and DC migration was tracked by magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT). Ex vivo analyses were performed to validate the imaging data. Results: DC labeling, both with MNPs and with 111In-oxine, did not affect DC phenotype or functionality. MRI and SPECT allowed the detection of iron and 111In in both axillary and popliteal LNs. Immunohistochemistry and γ-counting revealed the presence of DCs in LNs. Conclusions: MRI and SPECT imaging, by allowing in vivo dynamic monitoring of DC migration, could further the development and optimization of efficient anti-cancer vaccines.

Original languageEnglish
Pages (from-to)183-196
Number of pages14
JournalMolecular Imaging and Biology
Volume14
Issue number2
DOIs
Publication statusPublished - Apr 2012

Keywords

  • Breast cancer
  • Dendritic cells
  • In vivo imaging
  • MRI
  • SPECT
  • Vaccine protocol

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging

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