In vivo laser assisted end-to-end anastomosis with ICG-infused chitosan patches

Francesca Rossi, Paolo Matteini, Giuseppe Esposito, Alba Scerrati, Alessio Albanese, Alfredo Puca, Giulio Maira, Giacomo Rossi, Roberto Pini

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Laser assisted vascular repair is a new optimized technique based on the use of ICG-infused chitosan patch to close a vessel wound, with or even without few supporting single stitches. We present an in vivo experimental study on an innovative end-to-end laser assisted vascular anastomotic (LAVA) technique, performed with the application of ICGinfused chitosan patches. The photostability and the mechanical properties of ICG-infused chitosan films were preliminary measured. The in vivo study was performed in 10 New Zealand rabbits. After anesthesia, a 3-cm segment of the right common carotid artery was exposed, thus clamped proximally and distally. The artery was then interrupted by means of a full thickness cut. Three single microsutures were used to approximate the two vessel edges. The ICG-infused chitosan patch was rolled all over the anastomotic site and welded by the use of a diode laser emitting at 810 nm and equipped with a 300 μm diameter optical fiber. Welding was obtained by delivering single laser spots to induce local patch/tissue adhesion. The result was an immediate closure of the anastomosis, with no bleeding at clamps release. Thus animals underwent different follow-up periods, in order to evaluate the welded vessels over time. At follow-up examinations, all the anastomoses were patent and no bleeding signs were documented. Samples of welded vessels underwent histological examinations. Results showed that this technique offer several advantages over conventional suturing methods: simplification of the surgical procedure, shortening of the operative time, better re-endothelization and optimal vascular healing process.

Original languageEnglish
Title of host publicationOptics InfoBase Conference Papers
Publication statusPublished - 2011
EventEuropean Conference on Biomedical Optics, ECBO 2011 - Munich, Germany
Duration: May 22 2011May 26 2011


OtherEuropean Conference on Biomedical Optics, ECBO 2011


  • Bypass
  • Chitosan patch
  • Diode laser
  • End-to-end anastomosis
  • ICG
  • Vascular welding

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics


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