In vivo PC3 overexpression by retroviral vector affects cell differentiation of rat cortical precursors

Paolo Malatesta; Paola Sgadò; Luca Caneparo; Giuseppina Barsacchi; Federico Cremisi

doi:10.1016/S0165-3806(01)00170-5

In vivo PC3 overexpression by retroviral vector affects cell differentiation of rat cortical precursors

Paolo Malatesta, Paola Sgadò, Luca Caneparo, Giuseppina Barsacchi, Federico Cremisi

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article

Abstract

The PC3 gene is a marker of dividing neuroepithelial (NE) cells. We transduced single cortical precursors of the ventricular zone (VZ) with a PC3-carrying retroviral vector at E16 stage, and analysed the effects of transgene expression on their progeny in 3-week-old animals. Unlike control-transduced cells, all viable PC3-transduced cells remained close to the ventricle and displayed a round-shaped, undifferentiated morphology.

Original language	English
Pages (from-to)	181-185
Number of pages	5
Journal	Developmental Brain Research
Volume	128
Issue number	2
DOIs	https://doi.org/10.1016/S0165-3806(01)00170-5
Publication status	Published - Jun 29 2001

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Keywords

Asymmetric cell division
In vivo
Neural stem cell
Neurogenesis
PC3
Retroviral vector

ASJC Scopus subject areas

Developmental Biology
Developmental Neuroscience

Cite this

Malatesta, P., Sgadò, P., Caneparo, L., Barsacchi, G., & Cremisi, F. (2001). In vivo PC3 overexpression by retroviral vector affects cell differentiation of rat cortical precursors. Developmental Brain Research, 128(2), 181-185. https://doi.org/10.1016/S0165-3806(01)00170-5

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Access to Document

10.1016/S0165-3806(01)00170-5