In vivo PC3 overexpression by retroviral vector affects cell differentiation of rat cortical precursors

Paolo Malatesta; Paola Sgadò; Luca Caneparo; Giuseppina Barsacchi; Federico Cremisi

doi:10.1016/S0165-3806(01)00170-5

In vivo PC3 overexpression by retroviral vector affects cell differentiation of rat cortical precursors

Paolo Malatesta, Paola Sgadò, Luca Caneparo, Giuseppina Barsacchi, Federico Cremisi

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

The PC3 gene is a marker of dividing neuroepithelial (NE) cells. We transduced single cortical precursors of the ventricular zone (VZ) with a PC3-carrying retroviral vector at E16 stage, and analysed the effects of transgene expression on their progeny in 3-week-old animals. Unlike control-transduced cells, all viable PC3-transduced cells remained close to the ventricle and displayed a round-shaped, undifferentiated morphology.

Original language	English
Pages (from-to)	181-185
Number of pages	5
Journal	Developmental Brain Research
Volume	128
Issue number	2
DOIs	https://doi.org/10.1016/S0165-3806(01)00170-5
Publication status	Published - Jun 29 2001

Keywords

Asymmetric cell division
In vivo
Neural stem cell
Neurogenesis
PC3
Retroviral vector

ASJC Scopus subject areas

Developmental Biology
Developmental Neuroscience

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10.1016/S0165-3806(01)00170-5

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AU - Caneparo, Luca

AU - Barsacchi, Giuseppina

AU - Cremisi, Federico

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