In vivo quantitative magnetization transfer imaging correlates with histology during de- and remyelination in cuprizone-treated mice

Laura Turati, Marco Moscatelli, Alfonso Mastropietro, Nicholas G. Dowell, Ileana Zucca, Alessandra Erbetta, Chiara Cordiglieri, Greta Brenna, Beatrice Bianchi, Renato Mantegazza, Mara Cercignani, Fulvio Baggi, Ludovico Minati

Research output: Contribution to journalArticle

Abstract

The pool size ratio measured by quantitative magnetization transfer MRI is hypothesized to closely reflect myelin density, but their relationship has so far been confirmed mostly in ex vivo conditions. We investigate the correspondence between this parameter measured in vivo at 7.0T, with Black Gold II staining for myelin fibres, and with myelin basic protein and beta-tubulin immunofluorescence in a hybrid longitudinal study of C57BL/6 and SJL/J mice treated with cuprizone, a neurotoxicant causing relatively selective myelin loss followed by spontaneous remyelination upon treatment suspension. Our results confirm that pool size ratio measurements correlate with myelin content, with the correlation coefficient depending on strain and staining method, and demonstrate the in vivo applicability of this MRI technique to experimental mouse models of multiple sclerosis.

Original languageEnglish
Pages (from-to)327-337
Number of pages11
JournalNMR in Biomedicine
Volume28
Issue number3
DOIs
Publication statusPublished - Mar 1 2015

Keywords

  • Animal model
  • Cuprizone
  • Demyelination
  • Macromolecular pool size ratio (F)
  • Multiple sclerosis
  • Quantitative magnetization transfer imaging (qMT)
  • Remyelination

ASJC Scopus subject areas

  • Spectroscopy
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

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