In vivo quantitative magnetization transfer imaging correlates with histology during de- and remyelination in cuprizone-treated mice

Laura Turati, Marco Moscatelli, Alfonso Mastropietro, Nicholas G. Dowell, Ileana Zucca, Alessandra Erbetta, Chiara Cordiglieri, Greta Brenna, Beatrice Bianchi, Renato Mantegazza, Mara Cercignani, Fulvio Baggi, Ludovico Minati

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The pool size ratio measured by quantitative magnetization transfer MRI is hypothesized to closely reflect myelin density, but their relationship has so far been confirmed mostly in ex vivo conditions. We investigate the correspondence between this parameter measured in vivo at 7.0T, with Black Gold II staining for myelin fibres, and with myelin basic protein and beta-tubulin immunofluorescence in a hybrid longitudinal study of C57BL/6 and SJL/J mice treated with cuprizone, a neurotoxicant causing relatively selective myelin loss followed by spontaneous remyelination upon treatment suspension. Our results confirm that pool size ratio measurements correlate with myelin content, with the correlation coefficient depending on strain and staining method, and demonstrate the in vivo applicability of this MRI technique to experimental mouse models of multiple sclerosis.

Original languageEnglish
Pages (from-to)327-337
Number of pages11
JournalNMR in Biomedicine
Volume28
Issue number3
DOIs
Publication statusPublished - Mar 1 2015

Fingerprint

Cuprizone
Histology
Demyelinating Diseases
Myelin Sheath
Magnetic resonance imaging
Magnetization
Imaging techniques
Myelin Basic Protein
Tubulin
Suspensions
Staining and Labeling
Fibers
Multiple Sclerosis
Fluorescent Antibody Technique
Longitudinal Studies
Theoretical Models

Keywords

  • Animal model
  • Cuprizone
  • Demyelination
  • Macromolecular pool size ratio (F)
  • Multiple sclerosis
  • Quantitative magnetization transfer imaging (qMT)
  • Remyelination

ASJC Scopus subject areas

  • Spectroscopy
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

Cite this

In vivo quantitative magnetization transfer imaging correlates with histology during de- and remyelination in cuprizone-treated mice. / Turati, Laura; Moscatelli, Marco; Mastropietro, Alfonso; Dowell, Nicholas G.; Zucca, Ileana; Erbetta, Alessandra; Cordiglieri, Chiara; Brenna, Greta; Bianchi, Beatrice; Mantegazza, Renato; Cercignani, Mara; Baggi, Fulvio; Minati, Ludovico.

In: NMR in Biomedicine, Vol. 28, No. 3, 01.03.2015, p. 327-337.

Research output: Contribution to journalArticle

Turati, L, Moscatelli, M, Mastropietro, A, Dowell, NG, Zucca, I, Erbetta, A, Cordiglieri, C, Brenna, G, Bianchi, B, Mantegazza, R, Cercignani, M, Baggi, F & Minati, L 2015, 'In vivo quantitative magnetization transfer imaging correlates with histology during de- and remyelination in cuprizone-treated mice', NMR in Biomedicine, vol. 28, no. 3, pp. 327-337. https://doi.org/10.1002/nbm.3253
Turati, Laura ; Moscatelli, Marco ; Mastropietro, Alfonso ; Dowell, Nicholas G. ; Zucca, Ileana ; Erbetta, Alessandra ; Cordiglieri, Chiara ; Brenna, Greta ; Bianchi, Beatrice ; Mantegazza, Renato ; Cercignani, Mara ; Baggi, Fulvio ; Minati, Ludovico. / In vivo quantitative magnetization transfer imaging correlates with histology during de- and remyelination in cuprizone-treated mice. In: NMR in Biomedicine. 2015 ; Vol. 28, No. 3. pp. 327-337.
@article{36b9d4981a0a46d8a3b48932c8d16e0c,
title = "In vivo quantitative magnetization transfer imaging correlates with histology during de- and remyelination in cuprizone-treated mice",
abstract = "The pool size ratio measured by quantitative magnetization transfer MRI is hypothesized to closely reflect myelin density, but their relationship has so far been confirmed mostly in ex vivo conditions. We investigate the correspondence between this parameter measured in vivo at 7.0T, with Black Gold II staining for myelin fibres, and with myelin basic protein and beta-tubulin immunofluorescence in a hybrid longitudinal study of C57BL/6 and SJL/J mice treated with cuprizone, a neurotoxicant causing relatively selective myelin loss followed by spontaneous remyelination upon treatment suspension. Our results confirm that pool size ratio measurements correlate with myelin content, with the correlation coefficient depending on strain and staining method, and demonstrate the in vivo applicability of this MRI technique to experimental mouse models of multiple sclerosis.",
keywords = "Animal model, Cuprizone, Demyelination, Macromolecular pool size ratio (F), Multiple sclerosis, Quantitative magnetization transfer imaging (qMT), Remyelination",
author = "Laura Turati and Marco Moscatelli and Alfonso Mastropietro and Dowell, {Nicholas G.} and Ileana Zucca and Alessandra Erbetta and Chiara Cordiglieri and Greta Brenna and Beatrice Bianchi and Renato Mantegazza and Mara Cercignani and Fulvio Baggi and Ludovico Minati",
year = "2015",
month = "3",
day = "1",
doi = "10.1002/nbm.3253",
language = "English",
volume = "28",
pages = "327--337",
journal = "NMR in Biomedicine",
issn = "0952-3480",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

TY - JOUR

T1 - In vivo quantitative magnetization transfer imaging correlates with histology during de- and remyelination in cuprizone-treated mice

AU - Turati, Laura

AU - Moscatelli, Marco

AU - Mastropietro, Alfonso

AU - Dowell, Nicholas G.

AU - Zucca, Ileana

AU - Erbetta, Alessandra

AU - Cordiglieri, Chiara

AU - Brenna, Greta

AU - Bianchi, Beatrice

AU - Mantegazza, Renato

AU - Cercignani, Mara

AU - Baggi, Fulvio

AU - Minati, Ludovico

PY - 2015/3/1

Y1 - 2015/3/1

N2 - The pool size ratio measured by quantitative magnetization transfer MRI is hypothesized to closely reflect myelin density, but their relationship has so far been confirmed mostly in ex vivo conditions. We investigate the correspondence between this parameter measured in vivo at 7.0T, with Black Gold II staining for myelin fibres, and with myelin basic protein and beta-tubulin immunofluorescence in a hybrid longitudinal study of C57BL/6 and SJL/J mice treated with cuprizone, a neurotoxicant causing relatively selective myelin loss followed by spontaneous remyelination upon treatment suspension. Our results confirm that pool size ratio measurements correlate with myelin content, with the correlation coefficient depending on strain and staining method, and demonstrate the in vivo applicability of this MRI technique to experimental mouse models of multiple sclerosis.

AB - The pool size ratio measured by quantitative magnetization transfer MRI is hypothesized to closely reflect myelin density, but their relationship has so far been confirmed mostly in ex vivo conditions. We investigate the correspondence between this parameter measured in vivo at 7.0T, with Black Gold II staining for myelin fibres, and with myelin basic protein and beta-tubulin immunofluorescence in a hybrid longitudinal study of C57BL/6 and SJL/J mice treated with cuprizone, a neurotoxicant causing relatively selective myelin loss followed by spontaneous remyelination upon treatment suspension. Our results confirm that pool size ratio measurements correlate with myelin content, with the correlation coefficient depending on strain and staining method, and demonstrate the in vivo applicability of this MRI technique to experimental mouse models of multiple sclerosis.

KW - Animal model

KW - Cuprizone

KW - Demyelination

KW - Macromolecular pool size ratio (F)

KW - Multiple sclerosis

KW - Quantitative magnetization transfer imaging (qMT)

KW - Remyelination

UR - http://www.scopus.com/inward/record.url?scp=84924026903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924026903&partnerID=8YFLogxK

U2 - 10.1002/nbm.3253

DO - 10.1002/nbm.3253

M3 - Article

C2 - 25639498

AN - SCOPUS:84924026903

VL - 28

SP - 327

EP - 337

JO - NMR in Biomedicine

JF - NMR in Biomedicine

SN - 0952-3480

IS - 3

ER -