In vivo reactive neural plasticity investigation by means of correlative two photon: Electron microscopy

A. L. Allegra Mascaro, P. Cesare, L. Sacconi, G. Grasselli, G. Mandolesi, B. Maco, G. Knott, L. Huang, V. De Paola, P. Strata, F. S. Pavone

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In the adult nervous system, different populations of neurons correspond to different regenerative behavior. Although previous works showed that olivocerebellar fibers are capable of axonal regeneration in a suitable environment as a response to injury1, we have hitherto no details about the real dynamics of fiber regeneration. We set up a model of singularly axotomized climbing fibers (CF) to investigate their reparative properties in the adult central nervous system (CNS) in vivo. Time lapse two-photon imaging has been combined to laser nanosurgery2, 3 to define a temporal pattern of the degenerative event and to follow the structural rearrangement after injury. To characterize the damage and to elucidate the possible formation of new synaptic contacts on the sprouted branches of the lesioned CF, we combined two-photon in vivo imaging with block face scanning electron microscopy (FIB-SEM). Here we describe the approach followed to characterize the reactive plasticity after injury.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8588
DOIs
Publication statusPublished - 2013
EventMultiphoton Microscopy in the Biomedical Sciences XIII - San Francisco, CA, United States
Duration: Feb 3 2013Feb 5 2013

Other

OtherMultiphoton Microscopy in the Biomedical Sciences XIII
CountryUnited States
CitySan Francisco, CA
Period2/3/132/5/13

Keywords

  • Cerebellum
  • In vivo imaging
  • Laser nanosurgery
  • Two-photon microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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