In vivo restoration of T cell functions by human IL-1β or its 163-171 nonapeptide in immunodepressed mice

D. Frasca, D. Boraschi, S. Baschieri, P. Bossu, A. Tagliabue, L. Adorini, G. Doria

Research output: Contribution to journalArticle

Abstract

The immunorestorative capacities of human (hu) IL-1β or its synthetic fragment 163-171 (VQGEESNDK) were assessed in vivo in mice immunodepressed by aging, sublethal irradiation, or both. Subcutaneous administrtion of hu rIL-1β into immunodepressed animals immediately after carrier (horse red blood cells, HRBC) priming could restore to normal levels Th cell activity. This was measured as the ability of spleen cells from HRBC-primed mice to induce a hapten-specific antibody response in spleen cells from nonimmune mice in vitro stimulated with the hapten-carrier conjugate TNP-HRBC. In parallel, the ability of spleen cells from hu rIL-1β-treated immunodepressed animals to produce T cell growth factor activity upon in vitro mitogen stimulation was also increased significantly as compared to that of untreated mice and approached that of immunocompetent controls. The immunorestorative activity of hu rIL-1β on Th cell activity and T cell growth factor production could be mimicked by the synthetic nonapeptide 163-171 which, at the doses used, produced in most instances even greater effects than the whole protein. Although the optimal immunorestorative doses of the 163-171 peptide were several orders of magnitude higher than those of hu rIL-1β, the complete lack of IL-1-like inflammatory and toxic effects suggests that the synthetic hu IL-1β fragment may be successfully used as immunomodulating agent in the therapy of T cell immunodeficiencies.

Original languageEnglish
Pages (from-to)2651-2655
Number of pages5
JournalJournal of Immunology
Volume141
Issue number8
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Immunology

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    Frasca, D., Boraschi, D., Baschieri, S., Bossu, P., Tagliabue, A., Adorini, L., & Doria, G. (1988). In vivo restoration of T cell functions by human IL-1β or its 163-171 nonapeptide in immunodepressed mice. Journal of Immunology, 141(8), 2651-2655.