In vivo single voxel 1H MRS of glial brain tumors: Correlation with tissue histology and in vitro MRS

G. Barbarella, R. Ricci, G. Pirini, V. Tugnoli, M. R. Tosi, A. Bertoluzza, F. Calbucci, M. Leonardi, C. Trevisan, V. Eusebi

Research output: Contribution to journalArticle

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Abstract

Eighteen patients affected by glial brain tumors were examined by localized single voxel magnetic resonance spectroscopy (1H MRS). For all patients histological diagnosis was obtained after stereotactic biopsy or surgical removal of the tumor. Histological nuclear atypia, tumor cellularity, necrosis, vascular proliferation and percentage of Ki-67 positive cells in the total cell populations were evaluated. It was found that some of the in vivo 1H MRS parameters were related to the histological features of the tumor. In particular, the Cho(i)/Cr(i) metabolite ratio obtained from the neoplastic tissues paralleled the Ki-67 cell proliferation index. For some of the patients 1H and 13C in vitro high resolution magnetic spectroscopy of surgical specimens was also carried out. 1H MRS of aqueous extracts from different biopsy specimens of the same tumor furnished fine biochemical information on intratumor heterogeneity. The degree of histological vascular proliferation of the tumor was correlated to the presence of cholesteryl esters in the 13C spectrum of the lipid fraction of surgical specimens.

Original languageEnglish
Pages (from-to)461-468
Number of pages8
JournalInternational Journal of Oncology
Volume12
Issue number2
Publication statusPublished - Feb 1998

Fingerprint

Brain Neoplasms
Neuroglia
Histology
Neoplasms
Blood Vessels
Biopsy
Cholesterol Esters
Spectrum Analysis
Necrosis
Magnetic Resonance Spectroscopy
Cell Proliferation
In Vitro Techniques
Proton Magnetic Resonance Spectroscopy
Lipids
Population

Keywords

  • Brain tumors
  • Magnetic resonance spectroscopy
  • Tissue histology

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Barbarella, G., Ricci, R., Pirini, G., Tugnoli, V., Tosi, M. R., Bertoluzza, A., ... Eusebi, V. (1998). In vivo single voxel 1H MRS of glial brain tumors: Correlation with tissue histology and in vitro MRS. International Journal of Oncology, 12(2), 461-468.

In vivo single voxel 1H MRS of glial brain tumors : Correlation with tissue histology and in vitro MRS. / Barbarella, G.; Ricci, R.; Pirini, G.; Tugnoli, V.; Tosi, M. R.; Bertoluzza, A.; Calbucci, F.; Leonardi, M.; Trevisan, C.; Eusebi, V.

In: International Journal of Oncology, Vol. 12, No. 2, 02.1998, p. 461-468.

Research output: Contribution to journalArticle

Barbarella, G, Ricci, R, Pirini, G, Tugnoli, V, Tosi, MR, Bertoluzza, A, Calbucci, F, Leonardi, M, Trevisan, C & Eusebi, V 1998, 'In vivo single voxel 1H MRS of glial brain tumors: Correlation with tissue histology and in vitro MRS', International Journal of Oncology, vol. 12, no. 2, pp. 461-468.
Barbarella G, Ricci R, Pirini G, Tugnoli V, Tosi MR, Bertoluzza A et al. In vivo single voxel 1H MRS of glial brain tumors: Correlation with tissue histology and in vitro MRS. International Journal of Oncology. 1998 Feb;12(2):461-468.
Barbarella, G. ; Ricci, R. ; Pirini, G. ; Tugnoli, V. ; Tosi, M. R. ; Bertoluzza, A. ; Calbucci, F. ; Leonardi, M. ; Trevisan, C. ; Eusebi, V. / In vivo single voxel 1H MRS of glial brain tumors : Correlation with tissue histology and in vitro MRS. In: International Journal of Oncology. 1998 ; Vol. 12, No. 2. pp. 461-468.
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