'In vivo' studies on the pathophysiological mechanism of Vibrio parahaemolyticus TDH+-induced secretion

W. Baffone, A. Casaroli, R. Campana, B. Citterio, E. Vittoria, L. Pierfelici, G. Donelli

Research output: Contribution to journalArticlepeer-review

Abstract

The thermostable direct haemolysin (TDH) is considered to be the major virulence factors of Vibrio parahaemolyticus; however, poor information is available about its mechanism of action. In our study we examined the capacity of two V. parahaemolyticus TDH-producers (strains 2067 and 3305) to induce fluid secretion in rat ileal loop and to reveal the role of calcium ions (Ca 2+), calmodulin (CaM), and protein kinase C (PKC) in V. parahaemolyticus TDH+-induced fluid secretion. The results show that V. parahaemolyticus TDH+ strains were able to induce secretion in small intestine; on the contrary, this ability was not evidenced in the V. parahaemolyticus TDH- strain used as negative control. The data suggest an enterotoxic activity of haemolysin. Calcium ionophore A23187 and 1-verapamil (calcium channel blocker), when injected alone, induced fluid accumulation in the control loops. A further increase in fluid accumulation (P+ strains and a significant decrease (P2+ appears to be an important messenger involved in the stimulation of intestinal secretion, contrary to PKC and calmodulin which do not appear to have any role.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalMicrobial Pathogenesis
Volume38
Issue number2-3
DOIs
Publication statusPublished - Feb 2005

Keywords

  • Pathophysiological mechanism
  • TDH
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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