In vivo study of a technetium labelled anti-EGFr MoAb

Francesco Scopinaro; Giuseppe De Vincentis; Maria Banci; Orazio Schillaci; Mario Di Loreto; Roberta Danieli; Maria Ierardi; Anna Maria De Paola; Walter Gianni; Rita Massa

In vivo study of a technetium labelled anti-EGFr MoAb

Francesco Scopinaro, Giuseppe De Vincentis, Maria Banci, Orazio Schillaci, Mario Di Loreto, Roberta Danieli, Maria Ierardi, Anna Maria De Paola, Walter Gianni, Rita Massa

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Abstract

Epithelial Growth Factor receptors (EGFr) are normally present in all the epithelial cells, but their overexpression is closely related to presence of cancer. We have raised EGF-competitive antibody against EGFr and have labelled it with ¹³¹I and technetium. The ability of this antibody to bind to A431 cells to be internalized has been tested on A431 cells cultures. Its ability to give scintigraphic images of epithelial tumors has been tested on nu/nu balb c mice xenografted with A431 cells. The labelled antibody is well internalized by cultured cells. Xenografted tumors are clearly imaged both by ¹³¹I and ^99mTc anti EGFr MoAb. ^99mTc labelling is very interesting. The tumor/background ratio was 0.72 ± 0.2 for ^99mTc and 0.40 ± 0.6 ¹³¹I labelling. Moreover very high uptake of ^99mTc MoAb was obtained 2 hours after injection whereas the ¹³¹I antibody required 24 hours.

Original language	English
Pages (from-to)	1761-1765
Number of pages	5
Journal	Anticancer Research
Volume	17
Issue number	3 B
Publication status	Published - 1997

Keywords

Tc labelled MoAb
A 431 cells
Anti EGFr MoAb
Epidermal Growth Factor Receptor (EGFr)
Tumor bearing balb/c n/n mouse

ASJC Scopus subject areas

Cancer Research
Oncology

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title = "In vivo study of a technetium labelled anti-EGFr MoAb",

abstract = "Epithelial Growth Factor receptors (EGFr) are normally present in all the epithelial cells, but their overexpression is closely related to presence of cancer. We have raised EGF-competitive antibody against EGFr and have labelled it with 131I and technetium. The ability of this antibody to bind to A431 cells to be internalized has been tested on A431 cells cultures. Its ability to give scintigraphic images of epithelial tumors has been tested on nu/nu balb c mice xenografted with A431 cells. The labelled antibody is well internalized by cultured cells. Xenografted tumors are clearly imaged both by 131I and 99mTc anti EGFr MoAb. 99mTc labelling is very interesting. The tumor/background ratio was 0.72 ± 0.2 for 99mTc and 0.40 ± 0.6 131I labelling. Moreover very high uptake of 99mTc MoAb was obtained 2 hours after injection whereas the 131I antibody required 24 hours.",

keywords = "Tc labelled MoAb, A 431 cells, Anti EGFr MoAb, Epidermal Growth Factor Receptor (EGFr), Tumor bearing balb/c n/n mouse",

author = "Francesco Scopinaro and {De Vincentis}, Giuseppe and Maria Banci and Orazio Schillaci and {Di Loreto}, Mario and Roberta Danieli and Maria Ierardi and {De Paola}, {Anna Maria} and Walter Gianni and Rita Massa",

year = "1997",

language = "English",

volume = "17",

pages = "1761--1765",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "3 B",

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TY - JOUR

T1 - In vivo study of a technetium labelled anti-EGFr MoAb

AU - Scopinaro, Francesco

AU - De Vincentis, Giuseppe

AU - Banci, Maria

AU - Schillaci, Orazio

AU - Di Loreto, Mario

AU - Danieli, Roberta

AU - Ierardi, Maria

AU - De Paola, Anna Maria

AU - Gianni, Walter

AU - Massa, Rita

PY - 1997

Y1 - 1997

N2 - Epithelial Growth Factor receptors (EGFr) are normally present in all the epithelial cells, but their overexpression is closely related to presence of cancer. We have raised EGF-competitive antibody against EGFr and have labelled it with 131I and technetium. The ability of this antibody to bind to A431 cells to be internalized has been tested on A431 cells cultures. Its ability to give scintigraphic images of epithelial tumors has been tested on nu/nu balb c mice xenografted with A431 cells. The labelled antibody is well internalized by cultured cells. Xenografted tumors are clearly imaged both by 131I and 99mTc anti EGFr MoAb. 99mTc labelling is very interesting. The tumor/background ratio was 0.72 ± 0.2 for 99mTc and 0.40 ± 0.6 131I labelling. Moreover very high uptake of 99mTc MoAb was obtained 2 hours after injection whereas the 131I antibody required 24 hours.

AB - Epithelial Growth Factor receptors (EGFr) are normally present in all the epithelial cells, but their overexpression is closely related to presence of cancer. We have raised EGF-competitive antibody against EGFr and have labelled it with 131I and technetium. The ability of this antibody to bind to A431 cells to be internalized has been tested on A431 cells cultures. Its ability to give scintigraphic images of epithelial tumors has been tested on nu/nu balb c mice xenografted with A431 cells. The labelled antibody is well internalized by cultured cells. Xenografted tumors are clearly imaged both by 131I and 99mTc anti EGFr MoAb. 99mTc labelling is very interesting. The tumor/background ratio was 0.72 ± 0.2 for 99mTc and 0.40 ± 0.6 131I labelling. Moreover very high uptake of 99mTc MoAb was obtained 2 hours after injection whereas the 131I antibody required 24 hours.

KW - Tc labelled MoAb

KW - A 431 cells

KW - Anti EGFr MoAb

KW - Epidermal Growth Factor Receptor (EGFr)

KW - Tumor bearing balb/c n/n mouse

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