Incidence of chromosomes 1 and 17 aneusomy in breast cancer and adjacent tissue: An interphase cytogenetic study

Claudio Botti, Barbara Pescatore, Marcella Mottolese, Francesco Sciarretta, Claudia Greco, Franco Di Filippo, Giuseppe Maria Gandolfo, Francesco Cavaliere, Roberta Bovani, Antonio Varanese, Anna Maria Cianciulli

Research output: Contribution to journalArticle

Abstract

Background: Characterization of the biopathologic events underlying the early steps of breast carcinogenesis may have a dramatic impact on reducing breast cancer mortality. Genes involved in breast tumorigenesis are localized on chromosomes 1 and 17, and numeric aberrations of these chromosomes have been correlated with breast cancer tumorigenesis and progression. According to the field cancerization hypothesis, specific chromosome aberrations may be present in breast cancer and in normal-appearing adjacent tissue. The latter changes reflect the genomic damage that follows long-term carcinogenic exposure and precede the morphologically detectable neoplastic transformation. We hypothesize that detection of these aberrations in benign breast epithelium may provide a tool for molecular risk assessment. Study Design: Using fluorescence in situ hybridization with centromere-specific probes, we determined the status of chromosomes 1 and 17 in fresh imprints of 28 samples of primary tumors and 54 samples of their surrounding uninvolved parenchyma taken from patients undergoing operations for breast carcinoma. Ten contralateral breast biopsy specimens collected from patients with previous breast carcinoma were also evaluated as a surrogate of a high-risk group to rule out the hypothesis that chromosomal aneusomy in tumor-adjacent tissue could be related to a paracrine effect of the primary tumor. Ten samples of benign breast tissue taken from patients at low risk were used as controls to define tolerance limits for aneusomy definition. Results: Using threshold values of 40% of signal loss and 13% of signal gain to define chromosome aneusomy (ie, mean + 3 SDs of the control group signals), we found the following: 1) almost all primary breast tumors were aneusomic for chromosomes 1 and 17;2) primary breast tumor and adjacent uninvolved parenchyma shared the same pattern of chromosomes 1 and 17 aneusomy in 66.7% of patients; and 3) chromosomes 1 and 17 aneusomies in contralateral benign breast samples from high-risk patients were not different from those in primary breast tumor or adjacent tissue samples. Conclusions: These results suggest that chromosomes 1 and 17 aneusomy may represent an intermediate biomarker of breast tumorigenesis potentially useful to detect patients at high risk of breast carcinoma who may benefit from preventive interventions. (C) 2000 by the American College of Surgeons.

Original languageEnglish
Pages (from-to)530-539
Number of pages10
JournalJournal of the American College of Surgeons
Volume190
Issue number5
Publication statusPublished - May 2000

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Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 1
Interphase
Cytogenetics
Breast Neoplasms
Breast
Incidence
Carcinogenesis
Chromosome Aberrations
Neoplasms
Centromere
Fluorescence In Situ Hybridization
Epithelium
Chromosomes
Biomarkers
Biopsy
Control Groups
Mortality

ASJC Scopus subject areas

  • Surgery

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Botti, C., Pescatore, B., Mottolese, M., Sciarretta, F., Greco, C., Di Filippo, F., ... Cianciulli, A. M. (2000). Incidence of chromosomes 1 and 17 aneusomy in breast cancer and adjacent tissue: An interphase cytogenetic study. Journal of the American College of Surgeons, 190(5), 530-539.

Incidence of chromosomes 1 and 17 aneusomy in breast cancer and adjacent tissue : An interphase cytogenetic study. / Botti, Claudio; Pescatore, Barbara; Mottolese, Marcella; Sciarretta, Francesco; Greco, Claudia; Di Filippo, Franco; Gandolfo, Giuseppe Maria; Cavaliere, Francesco; Bovani, Roberta; Varanese, Antonio; Cianciulli, Anna Maria.

In: Journal of the American College of Surgeons, Vol. 190, No. 5, 05.2000, p. 530-539.

Research output: Contribution to journalArticle

Botti, C, Pescatore, B, Mottolese, M, Sciarretta, F, Greco, C, Di Filippo, F, Gandolfo, GM, Cavaliere, F, Bovani, R, Varanese, A & Cianciulli, AM 2000, 'Incidence of chromosomes 1 and 17 aneusomy in breast cancer and adjacent tissue: An interphase cytogenetic study', Journal of the American College of Surgeons, vol. 190, no. 5, pp. 530-539.
Botti, Claudio ; Pescatore, Barbara ; Mottolese, Marcella ; Sciarretta, Francesco ; Greco, Claudia ; Di Filippo, Franco ; Gandolfo, Giuseppe Maria ; Cavaliere, Francesco ; Bovani, Roberta ; Varanese, Antonio ; Cianciulli, Anna Maria. / Incidence of chromosomes 1 and 17 aneusomy in breast cancer and adjacent tissue : An interphase cytogenetic study. In: Journal of the American College of Surgeons. 2000 ; Vol. 190, No. 5. pp. 530-539.
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abstract = "Background: Characterization of the biopathologic events underlying the early steps of breast carcinogenesis may have a dramatic impact on reducing breast cancer mortality. Genes involved in breast tumorigenesis are localized on chromosomes 1 and 17, and numeric aberrations of these chromosomes have been correlated with breast cancer tumorigenesis and progression. According to the field cancerization hypothesis, specific chromosome aberrations may be present in breast cancer and in normal-appearing adjacent tissue. The latter changes reflect the genomic damage that follows long-term carcinogenic exposure and precede the morphologically detectable neoplastic transformation. We hypothesize that detection of these aberrations in benign breast epithelium may provide a tool for molecular risk assessment. Study Design: Using fluorescence in situ hybridization with centromere-specific probes, we determined the status of chromosomes 1 and 17 in fresh imprints of 28 samples of primary tumors and 54 samples of their surrounding uninvolved parenchyma taken from patients undergoing operations for breast carcinoma. Ten contralateral breast biopsy specimens collected from patients with previous breast carcinoma were also evaluated as a surrogate of a high-risk group to rule out the hypothesis that chromosomal aneusomy in tumor-adjacent tissue could be related to a paracrine effect of the primary tumor. Ten samples of benign breast tissue taken from patients at low risk were used as controls to define tolerance limits for aneusomy definition. Results: Using threshold values of 40{\%} of signal loss and 13{\%} of signal gain to define chromosome aneusomy (ie, mean + 3 SDs of the control group signals), we found the following: 1) almost all primary breast tumors were aneusomic for chromosomes 1 and 17;2) primary breast tumor and adjacent uninvolved parenchyma shared the same pattern of chromosomes 1 and 17 aneusomy in 66.7{\%} of patients; and 3) chromosomes 1 and 17 aneusomies in contralateral benign breast samples from high-risk patients were not different from those in primary breast tumor or adjacent tissue samples. Conclusions: These results suggest that chromosomes 1 and 17 aneusomy may represent an intermediate biomarker of breast tumorigenesis potentially useful to detect patients at high risk of breast carcinoma who may benefit from preventive interventions. (C) 2000 by the American College of Surgeons.",
author = "Claudio Botti and Barbara Pescatore and Marcella Mottolese and Francesco Sciarretta and Claudia Greco and {Di Filippo}, Franco and Gandolfo, {Giuseppe Maria} and Francesco Cavaliere and Roberta Bovani and Antonio Varanese and Cianciulli, {Anna Maria}",
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T1 - Incidence of chromosomes 1 and 17 aneusomy in breast cancer and adjacent tissue

T2 - An interphase cytogenetic study

AU - Botti, Claudio

AU - Pescatore, Barbara

AU - Mottolese, Marcella

AU - Sciarretta, Francesco

AU - Greco, Claudia

AU - Di Filippo, Franco

AU - Gandolfo, Giuseppe Maria

AU - Cavaliere, Francesco

AU - Bovani, Roberta

AU - Varanese, Antonio

AU - Cianciulli, Anna Maria

PY - 2000/5

Y1 - 2000/5

N2 - Background: Characterization of the biopathologic events underlying the early steps of breast carcinogenesis may have a dramatic impact on reducing breast cancer mortality. Genes involved in breast tumorigenesis are localized on chromosomes 1 and 17, and numeric aberrations of these chromosomes have been correlated with breast cancer tumorigenesis and progression. According to the field cancerization hypothesis, specific chromosome aberrations may be present in breast cancer and in normal-appearing adjacent tissue. The latter changes reflect the genomic damage that follows long-term carcinogenic exposure and precede the morphologically detectable neoplastic transformation. We hypothesize that detection of these aberrations in benign breast epithelium may provide a tool for molecular risk assessment. Study Design: Using fluorescence in situ hybridization with centromere-specific probes, we determined the status of chromosomes 1 and 17 in fresh imprints of 28 samples of primary tumors and 54 samples of their surrounding uninvolved parenchyma taken from patients undergoing operations for breast carcinoma. Ten contralateral breast biopsy specimens collected from patients with previous breast carcinoma were also evaluated as a surrogate of a high-risk group to rule out the hypothesis that chromosomal aneusomy in tumor-adjacent tissue could be related to a paracrine effect of the primary tumor. Ten samples of benign breast tissue taken from patients at low risk were used as controls to define tolerance limits for aneusomy definition. Results: Using threshold values of 40% of signal loss and 13% of signal gain to define chromosome aneusomy (ie, mean + 3 SDs of the control group signals), we found the following: 1) almost all primary breast tumors were aneusomic for chromosomes 1 and 17;2) primary breast tumor and adjacent uninvolved parenchyma shared the same pattern of chromosomes 1 and 17 aneusomy in 66.7% of patients; and 3) chromosomes 1 and 17 aneusomies in contralateral benign breast samples from high-risk patients were not different from those in primary breast tumor or adjacent tissue samples. Conclusions: These results suggest that chromosomes 1 and 17 aneusomy may represent an intermediate biomarker of breast tumorigenesis potentially useful to detect patients at high risk of breast carcinoma who may benefit from preventive interventions. (C) 2000 by the American College of Surgeons.

AB - Background: Characterization of the biopathologic events underlying the early steps of breast carcinogenesis may have a dramatic impact on reducing breast cancer mortality. Genes involved in breast tumorigenesis are localized on chromosomes 1 and 17, and numeric aberrations of these chromosomes have been correlated with breast cancer tumorigenesis and progression. According to the field cancerization hypothesis, specific chromosome aberrations may be present in breast cancer and in normal-appearing adjacent tissue. The latter changes reflect the genomic damage that follows long-term carcinogenic exposure and precede the morphologically detectable neoplastic transformation. We hypothesize that detection of these aberrations in benign breast epithelium may provide a tool for molecular risk assessment. Study Design: Using fluorescence in situ hybridization with centromere-specific probes, we determined the status of chromosomes 1 and 17 in fresh imprints of 28 samples of primary tumors and 54 samples of their surrounding uninvolved parenchyma taken from patients undergoing operations for breast carcinoma. Ten contralateral breast biopsy specimens collected from patients with previous breast carcinoma were also evaluated as a surrogate of a high-risk group to rule out the hypothesis that chromosomal aneusomy in tumor-adjacent tissue could be related to a paracrine effect of the primary tumor. Ten samples of benign breast tissue taken from patients at low risk were used as controls to define tolerance limits for aneusomy definition. Results: Using threshold values of 40% of signal loss and 13% of signal gain to define chromosome aneusomy (ie, mean + 3 SDs of the control group signals), we found the following: 1) almost all primary breast tumors were aneusomic for chromosomes 1 and 17;2) primary breast tumor and adjacent uninvolved parenchyma shared the same pattern of chromosomes 1 and 17 aneusomy in 66.7% of patients; and 3) chromosomes 1 and 17 aneusomies in contralateral benign breast samples from high-risk patients were not different from those in primary breast tumor or adjacent tissue samples. Conclusions: These results suggest that chromosomes 1 and 17 aneusomy may represent an intermediate biomarker of breast tumorigenesis potentially useful to detect patients at high risk of breast carcinoma who may benefit from preventive interventions. (C) 2000 by the American College of Surgeons.

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