Incomplete penetrance of NRXN1 deletions in families with schizophrenia

Giovanna Todarello, Ningping Feng, Bhaskar S. Kolachana, Chao Li, Radhakrishna Vakkalanka, Alessandro Bertolino, Daniel R. Weinberger, Richard E. Straub

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Neurexin 1 (NRXN1) is a presynaptic neuronal adhesion molecule that interacts with postsynaptic neuroligins in both glutamatergic and GABAergic synapses and is important in synaptic formation and function. NRXN1 deletions increase the risk of schizophrenia, so our aims were to explore this in our family sample, to distinguish de novo from inherited mutations, to examine transmission to affected and unaffected siblings and to estimate penetrance. We performed copy number analyses in NRXN1 using data from Illumina BeadArrays from 635 subjects with schizophrenia (276 in genotyped families), 487 of their unaffected parents and 309 unaffected siblings as well as 635 normal controls, all from the CBDB/NIMH Genetic Study of Schizophrenia. Deletions called by software were confirmed by quantitative PCR and comparative genome hybridization. There were deletions in 15 individuals in 11 families, including de novo exonic deletions in one case and one unaffected sibling. We observed no deletions in controls, 7 deletions in cases (1.10%), and an unexpectedly high deletion frequency in parents (n. = 5, 1.02%) and siblings (n. = 3, 0.97%). Three families showed inheritance from an unaffected parent, and in two families an unaffected parent did not transmit to the affected offspring. Thus we have added to the evidence that NRXN1 deletions are more frequent in patients with schizophrenia than in healthy individuals. However, the presence of de novo deletions in unaffected relatives and transmission from and to unaffected family members demonstrated that while the deletions may well have been necessary for some carriers to develop schizophrenia, they were not always sufficient.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalSchizophrenia Research
Volume155
Issue number1-3
DOIs
Publication statusPublished - 2014

Fingerprint

Penetrance
Schizophrenia
Siblings
Parents
National Institute of Mental Health (U.S.)
Comparative Genomic Hybridization
Synapses
Software
Polymerase Chain Reaction
Mutation

Keywords

  • CNV
  • Copy number variation
  • Deletion
  • Neurexin
  • NRXN1
  • Schizophrenia

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Biological Psychiatry
  • Medicine(all)

Cite this

Todarello, G., Feng, N., Kolachana, B. S., Li, C., Vakkalanka, R., Bertolino, A., ... Straub, R. E. (2014). Incomplete penetrance of NRXN1 deletions in families with schizophrenia. Schizophrenia Research, 155(1-3), 1-7. https://doi.org/10.1016/j.schres.2014.02.023

Incomplete penetrance of NRXN1 deletions in families with schizophrenia. / Todarello, Giovanna; Feng, Ningping; Kolachana, Bhaskar S.; Li, Chao; Vakkalanka, Radhakrishna; Bertolino, Alessandro; Weinberger, Daniel R.; Straub, Richard E.

In: Schizophrenia Research, Vol. 155, No. 1-3, 2014, p. 1-7.

Research output: Contribution to journalArticle

Todarello, G, Feng, N, Kolachana, BS, Li, C, Vakkalanka, R, Bertolino, A, Weinberger, DR & Straub, RE 2014, 'Incomplete penetrance of NRXN1 deletions in families with schizophrenia', Schizophrenia Research, vol. 155, no. 1-3, pp. 1-7. https://doi.org/10.1016/j.schres.2014.02.023
Todarello G, Feng N, Kolachana BS, Li C, Vakkalanka R, Bertolino A et al. Incomplete penetrance of NRXN1 deletions in families with schizophrenia. Schizophrenia Research. 2014;155(1-3):1-7. https://doi.org/10.1016/j.schres.2014.02.023
Todarello, Giovanna ; Feng, Ningping ; Kolachana, Bhaskar S. ; Li, Chao ; Vakkalanka, Radhakrishna ; Bertolino, Alessandro ; Weinberger, Daniel R. ; Straub, Richard E. / Incomplete penetrance of NRXN1 deletions in families with schizophrenia. In: Schizophrenia Research. 2014 ; Vol. 155, No. 1-3. pp. 1-7.
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abstract = "Neurexin 1 (NRXN1) is a presynaptic neuronal adhesion molecule that interacts with postsynaptic neuroligins in both glutamatergic and GABAergic synapses and is important in synaptic formation and function. NRXN1 deletions increase the risk of schizophrenia, so our aims were to explore this in our family sample, to distinguish de novo from inherited mutations, to examine transmission to affected and unaffected siblings and to estimate penetrance. We performed copy number analyses in NRXN1 using data from Illumina BeadArrays from 635 subjects with schizophrenia (276 in genotyped families), 487 of their unaffected parents and 309 unaffected siblings as well as 635 normal controls, all from the CBDB/NIMH Genetic Study of Schizophrenia. Deletions called by software were confirmed by quantitative PCR and comparative genome hybridization. There were deletions in 15 individuals in 11 families, including de novo exonic deletions in one case and one unaffected sibling. We observed no deletions in controls, 7 deletions in cases (1.10{\%}), and an unexpectedly high deletion frequency in parents (n. = 5, 1.02{\%}) and siblings (n. = 3, 0.97{\%}). Three families showed inheritance from an unaffected parent, and in two families an unaffected parent did not transmit to the affected offspring. Thus we have added to the evidence that NRXN1 deletions are more frequent in patients with schizophrenia than in healthy individuals. However, the presence of de novo deletions in unaffected relatives and transmission from and to unaffected family members demonstrated that while the deletions may well have been necessary for some carriers to develop schizophrenia, they were not always sufficient.",
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