Incorporation of chromosomal proteins HMG-14/ HMG-17 into nascent nucleosomes induces an extended chromatin conformation and enhances the utilization of active transcription complexes

Lothar Trieschmann, Pedro J. Alfonso, Massimo P. Crippa, Alan P. Wolffe, Michael Bustin

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The role of chromosomal proteins HMG-14 and HMG-17 in the generation of transcriptionally active chromatin was studied in a Xenopus laevis egg extract which supports complementary DNA strand synthesis and chromatin assembly. Chromosomal proteins HMG14/HMG-17 enhanced transcription from a chromatin template carrying a 5S rRNA gene, but not from a DNA template. The transcriptional potential of chromatin was enhanced only when these proteins were incorporated into the template during, but not after, chromatin assembly. HMG-14 and HMG-17 stimulate transcription by increasing the activity, and not the number, of transcribed templates. They unfold the chromatin template without affecting the nucleosomal repeat or decreasing the content of histone B4. We suggest that HMG-14/HMG-17 enhance transcription by inducing an extended conformation in the chromatin fiber, perhaps due to interactions with histone tails in nucleosomes. By disrupting the higher order chromatin structure HMG-14/HMG-17 increase the accessibility of target sequences to components of the transcriptional apparatus.

Original languageEnglish
Pages (from-to)1478-1489
Number of pages12
JournalEMBO Journal
Volume14
Issue number7
Publication statusPublished - 1995

Fingerprint

HMGN2 Protein
HMGN1 Protein
Nucleosomes
Transcription
Chromatin
Conformations
Chromatin Assembly and Disassembly
Histones
Xenopus laevis
rRNA Genes
Ovum
Tail
Complementary DNA
Genes
DNA

Keywords

  • Active chromatin
  • Chromatin assembly
  • Chromosomal proteins
  • Transcription
  • Xenopus

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Incorporation of chromosomal proteins HMG-14/ HMG-17 into nascent nucleosomes induces an extended chromatin conformation and enhances the utilization of active transcription complexes. / Trieschmann, Lothar; Alfonso, Pedro J.; Crippa, Massimo P.; Wolffe, Alan P.; Bustin, Michael.

In: EMBO Journal, Vol. 14, No. 7, 1995, p. 1478-1489.

Research output: Contribution to journalArticle

@article{9a287beaa1d54293abe1e0e3e2d6b996,
title = "Incorporation of chromosomal proteins HMG-14/ HMG-17 into nascent nucleosomes induces an extended chromatin conformation and enhances the utilization of active transcription complexes",
abstract = "The role of chromosomal proteins HMG-14 and HMG-17 in the generation of transcriptionally active chromatin was studied in a Xenopus laevis egg extract which supports complementary DNA strand synthesis and chromatin assembly. Chromosomal proteins HMG14/HMG-17 enhanced transcription from a chromatin template carrying a 5S rRNA gene, but not from a DNA template. The transcriptional potential of chromatin was enhanced only when these proteins were incorporated into the template during, but not after, chromatin assembly. HMG-14 and HMG-17 stimulate transcription by increasing the activity, and not the number, of transcribed templates. They unfold the chromatin template without affecting the nucleosomal repeat or decreasing the content of histone B4. We suggest that HMG-14/HMG-17 enhance transcription by inducing an extended conformation in the chromatin fiber, perhaps due to interactions with histone tails in nucleosomes. By disrupting the higher order chromatin structure HMG-14/HMG-17 increase the accessibility of target sequences to components of the transcriptional apparatus.",
keywords = "Active chromatin, Chromatin assembly, Chromosomal proteins, Transcription, Xenopus",
author = "Lothar Trieschmann and Alfonso, {Pedro J.} and Crippa, {Massimo P.} and Wolffe, {Alan P.} and Michael Bustin",
year = "1995",
language = "English",
volume = "14",
pages = "1478--1489",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "7",

}

TY - JOUR

T1 - Incorporation of chromosomal proteins HMG-14/ HMG-17 into nascent nucleosomes induces an extended chromatin conformation and enhances the utilization of active transcription complexes

AU - Trieschmann, Lothar

AU - Alfonso, Pedro J.

AU - Crippa, Massimo P.

AU - Wolffe, Alan P.

AU - Bustin, Michael

PY - 1995

Y1 - 1995

N2 - The role of chromosomal proteins HMG-14 and HMG-17 in the generation of transcriptionally active chromatin was studied in a Xenopus laevis egg extract which supports complementary DNA strand synthesis and chromatin assembly. Chromosomal proteins HMG14/HMG-17 enhanced transcription from a chromatin template carrying a 5S rRNA gene, but not from a DNA template. The transcriptional potential of chromatin was enhanced only when these proteins were incorporated into the template during, but not after, chromatin assembly. HMG-14 and HMG-17 stimulate transcription by increasing the activity, and not the number, of transcribed templates. They unfold the chromatin template without affecting the nucleosomal repeat or decreasing the content of histone B4. We suggest that HMG-14/HMG-17 enhance transcription by inducing an extended conformation in the chromatin fiber, perhaps due to interactions with histone tails in nucleosomes. By disrupting the higher order chromatin structure HMG-14/HMG-17 increase the accessibility of target sequences to components of the transcriptional apparatus.

AB - The role of chromosomal proteins HMG-14 and HMG-17 in the generation of transcriptionally active chromatin was studied in a Xenopus laevis egg extract which supports complementary DNA strand synthesis and chromatin assembly. Chromosomal proteins HMG14/HMG-17 enhanced transcription from a chromatin template carrying a 5S rRNA gene, but not from a DNA template. The transcriptional potential of chromatin was enhanced only when these proteins were incorporated into the template during, but not after, chromatin assembly. HMG-14 and HMG-17 stimulate transcription by increasing the activity, and not the number, of transcribed templates. They unfold the chromatin template without affecting the nucleosomal repeat or decreasing the content of histone B4. We suggest that HMG-14/HMG-17 enhance transcription by inducing an extended conformation in the chromatin fiber, perhaps due to interactions with histone tails in nucleosomes. By disrupting the higher order chromatin structure HMG-14/HMG-17 increase the accessibility of target sequences to components of the transcriptional apparatus.

KW - Active chromatin

KW - Chromatin assembly

KW - Chromosomal proteins

KW - Transcription

KW - Xenopus

UR - http://www.scopus.com/inward/record.url?scp=0028958663&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028958663&partnerID=8YFLogxK

M3 - Article

C2 - 7729423

AN - SCOPUS:0028958663

VL - 14

SP - 1478

EP - 1489

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 7

ER -