Increase in α-synuclein immunoreactivity during long-term synaptic plasticity

F. Battaglia, P. Girlanda, G. Vita, A. Toscano, I. Antonova, R. D. Hawkins, O. Arancio

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Previous research suggests that the cytosolic protein, α-synuclein, is involved in neurotransmitter release and plasticity in the central nervous system (Clayton et al, TINS 21:249, 1998; Abeliovich et al, Neuron 25: 239, 2000). However, the mechanisms of this involvement are poorly understood. We have begun to address this question in cultured hippocampal neurons, where glutamate produces long-lasting increases in mEPSC frequency, EPSC amplitude, and the number of synaptophysin-immunoreactive puncta (Antonova et al, Soc. Neurosci. Abstr. 25: 733, 1999; Malgaroli et al, Nature 357:134, 1992). Therefore, we examined the effects of glutamate application on α-synuclein immunoreactivity. Under control conditions, α-synuclein immunoreactivity appeared in puncta along the processes and also throughout the cell body. Double staining for α-synuclein and the presynaptic vesicle-associated protein synapsin I showed that approximately 51% of the α-synuclein-immunoreactive puncta colocalized with synapsin 1-immunoreactive puncta. After a 1-min. glutamate application (200 μM), the number of α-synuclein-immunoreactive puncta increased significantly (41.8%±9.1, mean±SEM, P

Original languageEnglish
JournalNeurological Sciences
Issue number4 SUPPL.
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology


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