Increase in chromogranin A- and serotonin-positive cells in pouch mucosa of patients with ulcerative colitis undergoing proctocolectomy

Paolo Giuffrida, Alessandro Vanoli, Elena Biletta, Catherine Klersy, Gaetano Bergamaschi, Gianluca M. Sampietro, Sandro Ardizzone, Ombretta Luinetti, Michele Martino, Marco Vincenzo Lenti, Nicola Aronico, Marco Curti, Rachele Manca, Paolo Fociani, Marta Truffi, Fabio Corsi, Gino Roberto Corazza, Antonio Di Sabatino

Research output: Contribution to journalArticle

Abstract

Background: Inflammatory bowel disease (IBD) is associated with neuroendocrine cell hyperplasia. Aims: We investigated neuroendocrine cells in J-pouches of patients with ulcerative colitis undergoing restorative proctocolectomy and ileal pouch-anal anastomosis. Methods: Sections from pouch biopsies of 17 patients and ileal biopsies of 17 active IBD patients and 16 controls were processed by immunohistochemistry for chromogranin A (CgA) and serotonin. Mucosal tryptophan hydroxylase (TpH)-1 and serotonin-selective reuptake transporter (SERT) transcripts were measured by quantitative RT-PCR. TpH-1 and SERT transcripts were detected in pouch biopsies cultured with infliximab or its isotype control, while interleukin (IL)-6 and IL-8 were measured in biopsy supernatants. Results: A significant increase in CgA-positive cells and serotonin-positive cells was observed in both pouch and IBD ileum compared to control ileum. Significantly raised transcripts of TpH-1, but not SERT, were found in IBD ileum in comparison to control ileum, with no significant difference between pouch and IBD ileum. Infliximab had no influence on ex vivo pouch expression of TpH-1 and SERT, nor on the production of IL-6 and IL-8. Conclusion: We here demonstrated neuroendocrine cell hyperplasia in pouch mucosa. Further studies are needed to clarify the pathophysiological implication of this finding.

Original languageEnglish
JournalDigestive and Liver Disease
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Chromogranin A
Tryptophan Hydroxylase
Ulcerative Colitis
Ileum
Serotonin
Mucous Membrane
Inflammatory Bowel Diseases
Neuroendocrine Cells
Biopsy
Colonic Pouches
Interleukin-8
Hyperplasia
Interleukin-6
Restorative Proctocolectomy
Immunohistochemistry
Polymerase Chain Reaction

Keywords

  • Chromogranin A
  • Crohn's disease
  • Pouchitis
  • Serotonin
  • Ulcerative colitis

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

Increase in chromogranin A- and serotonin-positive cells in pouch mucosa of patients with ulcerative colitis undergoing proctocolectomy. / Giuffrida, Paolo; Vanoli, Alessandro; Biletta, Elena; Klersy, Catherine; Bergamaschi, Gaetano; Sampietro, Gianluca M.; Ardizzone, Sandro; Luinetti, Ombretta; Martino, Michele; Lenti, Marco Vincenzo; Aronico, Nicola; Curti, Marco; Manca, Rachele; Fociani, Paolo; Truffi, Marta; Corsi, Fabio; Corazza, Gino Roberto; Di Sabatino, Antonio.

In: Digestive and Liver Disease, 01.01.2018.

Research output: Contribution to journalArticle

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title = "Increase in chromogranin A- and serotonin-positive cells in pouch mucosa of patients with ulcerative colitis undergoing proctocolectomy",
abstract = "Background: Inflammatory bowel disease (IBD) is associated with neuroendocrine cell hyperplasia. Aims: We investigated neuroendocrine cells in J-pouches of patients with ulcerative colitis undergoing restorative proctocolectomy and ileal pouch-anal anastomosis. Methods: Sections from pouch biopsies of 17 patients and ileal biopsies of 17 active IBD patients and 16 controls were processed by immunohistochemistry for chromogranin A (CgA) and serotonin. Mucosal tryptophan hydroxylase (TpH)-1 and serotonin-selective reuptake transporter (SERT) transcripts were measured by quantitative RT-PCR. TpH-1 and SERT transcripts were detected in pouch biopsies cultured with infliximab or its isotype control, while interleukin (IL)-6 and IL-8 were measured in biopsy supernatants. Results: A significant increase in CgA-positive cells and serotonin-positive cells was observed in both pouch and IBD ileum compared to control ileum. Significantly raised transcripts of TpH-1, but not SERT, were found in IBD ileum in comparison to control ileum, with no significant difference between pouch and IBD ileum. Infliximab had no influence on ex vivo pouch expression of TpH-1 and SERT, nor on the production of IL-6 and IL-8. Conclusion: We here demonstrated neuroendocrine cell hyperplasia in pouch mucosa. Further studies are needed to clarify the pathophysiological implication of this finding.",
keywords = "Chromogranin A, Crohn's disease, Pouchitis, Serotonin, Ulcerative colitis",
author = "Paolo Giuffrida and Alessandro Vanoli and Elena Biletta and Catherine Klersy and Gaetano Bergamaschi and Sampietro, {Gianluca M.} and Sandro Ardizzone and Ombretta Luinetti and Michele Martino and Lenti, {Marco Vincenzo} and Nicola Aronico and Marco Curti and Rachele Manca and Paolo Fociani and Marta Truffi and Fabio Corsi and Corazza, {Gino Roberto} and {Di Sabatino}, Antonio",
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month = "1",
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T1 - Increase in chromogranin A- and serotonin-positive cells in pouch mucosa of patients with ulcerative colitis undergoing proctocolectomy

AU - Giuffrida, Paolo

AU - Vanoli, Alessandro

AU - Biletta, Elena

AU - Klersy, Catherine

AU - Bergamaschi, Gaetano

AU - Sampietro, Gianluca M.

AU - Ardizzone, Sandro

AU - Luinetti, Ombretta

AU - Martino, Michele

AU - Lenti, Marco Vincenzo

AU - Aronico, Nicola

AU - Curti, Marco

AU - Manca, Rachele

AU - Fociani, Paolo

AU - Truffi, Marta

AU - Corsi, Fabio

AU - Corazza, Gino Roberto

AU - Di Sabatino, Antonio

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Inflammatory bowel disease (IBD) is associated with neuroendocrine cell hyperplasia. Aims: We investigated neuroendocrine cells in J-pouches of patients with ulcerative colitis undergoing restorative proctocolectomy and ileal pouch-anal anastomosis. Methods: Sections from pouch biopsies of 17 patients and ileal biopsies of 17 active IBD patients and 16 controls were processed by immunohistochemistry for chromogranin A (CgA) and serotonin. Mucosal tryptophan hydroxylase (TpH)-1 and serotonin-selective reuptake transporter (SERT) transcripts were measured by quantitative RT-PCR. TpH-1 and SERT transcripts were detected in pouch biopsies cultured with infliximab or its isotype control, while interleukin (IL)-6 and IL-8 were measured in biopsy supernatants. Results: A significant increase in CgA-positive cells and serotonin-positive cells was observed in both pouch and IBD ileum compared to control ileum. Significantly raised transcripts of TpH-1, but not SERT, were found in IBD ileum in comparison to control ileum, with no significant difference between pouch and IBD ileum. Infliximab had no influence on ex vivo pouch expression of TpH-1 and SERT, nor on the production of IL-6 and IL-8. Conclusion: We here demonstrated neuroendocrine cell hyperplasia in pouch mucosa. Further studies are needed to clarify the pathophysiological implication of this finding.

AB - Background: Inflammatory bowel disease (IBD) is associated with neuroendocrine cell hyperplasia. Aims: We investigated neuroendocrine cells in J-pouches of patients with ulcerative colitis undergoing restorative proctocolectomy and ileal pouch-anal anastomosis. Methods: Sections from pouch biopsies of 17 patients and ileal biopsies of 17 active IBD patients and 16 controls were processed by immunohistochemistry for chromogranin A (CgA) and serotonin. Mucosal tryptophan hydroxylase (TpH)-1 and serotonin-selective reuptake transporter (SERT) transcripts were measured by quantitative RT-PCR. TpH-1 and SERT transcripts were detected in pouch biopsies cultured with infliximab or its isotype control, while interleukin (IL)-6 and IL-8 were measured in biopsy supernatants. Results: A significant increase in CgA-positive cells and serotonin-positive cells was observed in both pouch and IBD ileum compared to control ileum. Significantly raised transcripts of TpH-1, but not SERT, were found in IBD ileum in comparison to control ileum, with no significant difference between pouch and IBD ileum. Infliximab had no influence on ex vivo pouch expression of TpH-1 and SERT, nor on the production of IL-6 and IL-8. Conclusion: We here demonstrated neuroendocrine cell hyperplasia in pouch mucosa. Further studies are needed to clarify the pathophysiological implication of this finding.

KW - Chromogranin A

KW - Crohn's disease

KW - Pouchitis

KW - Serotonin

KW - Ulcerative colitis

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U2 - 10.1016/j.dld.2018.04.021

DO - 10.1016/j.dld.2018.04.021

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JO - Digestive and Liver Disease

JF - Digestive and Liver Disease

SN - 1590-8658

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