Increase of dopamine β-hydroxylase immunoreactivity in non-noradrenergic nerves of rat cerebral arteries following long-term sympathectomy

M. C. Mione, G. Sancesario, V. D'Angelo, G. Bernardi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The expression of dopamine β-hydroxylase (DBH) and tyrosine hydroxylase (TH) immunoreactivity (IR) after short-term (2 days) and long-term (3 weeks) sympathectomy was investigated in rat cerebral vessels, dura mater and pterygopalatine ganglion neurones (which are known to project to cerebral arteries) by immunohistochemistry at both the light and electron microscopical levels. TH-IR, like glyoxylic acid-induced fluorescence, was completely abolished by sympathectomy. By contrast, DBH-IR was localized in nerve fibres, lacking 5-hydroxydopamine (5-OHDA)-labelled vesicles, along cerebral vessels of long-term sympathectomized rats, but not in the dura mater, and in ptergopalatine ganglia, where the number of DBH-IR neurons increased from 27.87% to 54.11%. Since virtually all the pterygopalatine neurons displayed choline acetyltransferase (ChAT)-IR, both in control and sympathectomized rats, it is concluded that long-term sympathectomy caused an increase of the expression of DBH-IR in cholinergic neurones of the pterygopalatine ganglion, without these neurons producing or storing noradrenaline.

Original languageEnglish
Pages (from-to)167-171
Number of pages5
JournalNeuroscience Letters
Volume123
Issue number2
DOIs
Publication statusPublished - Feb 25 1991

Fingerprint

Sympathectomy
Cerebral Arteries
Mixed Function Oxygenases
Dopamine
Parasympathetic Ganglia
Neurons
Dura Mater
Tyrosine 3-Monooxygenase
Cholinergic Neurons
Choline O-Acetyltransferase
Nerve Fibers
Ganglia
Norepinephrine
Fluorescence
Immunohistochemistry
Electrons
Light

Keywords

  • Cerebral vessels
  • Dopamine β-hydroxylase
  • Immunohistochemistry
  • Long-term sympathectomy
  • Pterygopalatine ganglion
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Increase of dopamine β-hydroxylase immunoreactivity in non-noradrenergic nerves of rat cerebral arteries following long-term sympathectomy. / Mione, M. C.; Sancesario, G.; D'Angelo, V.; Bernardi, G.

In: Neuroscience Letters, Vol. 123, No. 2, 25.02.1991, p. 167-171.

Research output: Contribution to journalArticle

Mione, M. C. ; Sancesario, G. ; D'Angelo, V. ; Bernardi, G. / Increase of dopamine β-hydroxylase immunoreactivity in non-noradrenergic nerves of rat cerebral arteries following long-term sympathectomy. In: Neuroscience Letters. 1991 ; Vol. 123, No. 2. pp. 167-171.
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