Increase of n-NOS and i-NOS in Rat Colon After Sacral Neuromodulation

Roberto Ghiselli, Guendalina Lucarini, Fiorenza Orlando, Monica Ortenzi, Luca Cardinali, Mauro Provinciali, Roberto Di Primio, Mario Guerrieri

Research output: Contribution to journalArticle

Abstract

Objective: Sacral neuromodulation (SNM) is proposed to treat different anorectal dysfunctions but its mechanism of action is not yet known. Our previous study demonstrated how SNM can significantly increase neuronal nitric oxide synthase NOS (n-NOS) and inducible NOS (i-NOS) expression in the anus and rectum of rats. There are no reports regarding the relation between SNM and NOS in colonic cells: our aim was to assess NOS expression in colonic rat model after SNM. Materials and Methods: Twenty-six female Sprangue-Dawley rats were considered: group I, normal control rats; group II, sham treatment rats, in whom electrodes for electrical stimulation were placed in S1 foramen bilaterally and left in place, without performing neuromodulation; group III, rats in whom SNM was performed. After 14 days, the rats were sacrificed and we evaluated n-NOS and i-NOS in colonic specimens by immunohistochemistry and Western Blot analysis. Results: Western Blot analysis showed that levels of n-NOS and i-NOS were higher in colon of the III group rats respect to the others; in particular, immunohistochemistry revealed that, after neuromodulation, n-NOS expression in the muscle cells and i-NOS expression in glandular epithelium and nervous cells were highly represented (p < 0.05). Conclusion: Our study showed that in colon, SNM is able to influence NO synthesis, activating n-NOS expression in muscle cells and i-NOS expression in glandular epithelium and nervous cells. Our study showed a complex colonic response to SNM. This experimental model could be applied to better understand the mechanism of action of SNM in bowel dysfunction.

Original languageEnglish
Pages (from-to)761-766
Number of pages6
JournalNeuromodulation
Volume20
Issue number8
DOIs
Publication statusPublished - Dec 1 2017

Keywords

  • Animal model
  • bowel dysfunction
  • colon
  • neuromodulation
  • nitric oxide

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

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    Ghiselli, R., Lucarini, G., Orlando, F., Ortenzi, M., Cardinali, L., Provinciali, M., Di Primio, R., & Guerrieri, M. (2017). Increase of n-NOS and i-NOS in Rat Colon After Sacral Neuromodulation. Neuromodulation, 20(8), 761-766. https://doi.org/10.1111/ner.12644