Abstract
In the present study we measured PC-1 content, tumour necrosis factor (TNF)-α gene expression, and insulin stimulation of insulin receptor tyrosine-kinase activity in adipose tissue from nonobese, non-diabetic subjects. These parameters were correlated with in vivo insulin action as measured by the intravenous insulin tolerance test K(itt) values). PC-1 content was negatively correlated with K(itt) values (r = -0.5, p = 0.04) and positively with plasma insulin levels both fasting (r = 0.58, p = 0.009) and after 120 min during oral glucose tolerance test (OGTT) (r = 0.67, p = 0.002). Moreover, adipose tissue PC-1 content: was higher in relatively insulin-resistant subjects (K(itt) values lower than 6) than in relatively insulin-sensitive subjects (K(itt) values higher than 6) (525±49 ng/mg protein vs 33±45, respectively, p = 0.012). Adipose tissue insulin receptor tyrosine kinase activity in response to insulin was significantly lower at all insulin concentrations tested p = 0.017, by two-way analysis of variance test) in insulin-resistant than in insulin-sensitive subjects (K(itt) values lower higher than 6, respectively). In contrast to PC-1, no significant correlation was observed between adipose tissue TNF-α mRNA content and K(itt) values, and plasma insulin levels, both fasting and at after 120 min during OGTT. Also, no difference was observed in TNF-α mRNA content between subjects with K(itt) values higher or lower than 6. These studies in adipose tissue, together with our previous studies in skeletal muscle raise the possibility that PC-1, by regulating insulin receptor function, may play a role in the degree of insulin sensitivity in non-obese, nondiabetic subjects.
| Original language | English |
|---|---|
| Pages (from-to) | 282-289 |
| Number of pages | 8 |
| Journal | Diabetologia |
| Volume | 40 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1997 |
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Keywords
- Adipose tissue
- Insulin receptor tyrosine-kinase inhibitors
- Insulin sensitivity
- Insulin tolerance test
- PC-1
- Tumor necrosis factor-α
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Internal Medicine
Cite this
Increased adipose tissue PC-1 protein content, but not tumour necrosis factor-α gene expression, is associated with a reduction of both whole body insulin sensitivity and insulin receptor tyrosine-kinase activity. / Frittitta, L.; Youngren, J. F.; Sbraccia, P.; D'Adamo, M.; Buongiorno, A.; Vigneri, R.; Goldfine, I. D.; Trischitta, V.
In: Diabetologia, Vol. 40, No. 3, 1997, p. 282-289.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Increased adipose tissue PC-1 protein content, but not tumour necrosis factor-α gene expression, is associated with a reduction of both whole body insulin sensitivity and insulin receptor tyrosine-kinase activity
AU - Frittitta, L.
AU - Youngren, J. F.
AU - Sbraccia, P.
AU - D'Adamo, M.
AU - Buongiorno, A.
AU - Vigneri, R.
AU - Goldfine, I. D.
AU - Trischitta, V.
PY - 1997
Y1 - 1997
N2 - In the present study we measured PC-1 content, tumour necrosis factor (TNF)-α gene expression, and insulin stimulation of insulin receptor tyrosine-kinase activity in adipose tissue from nonobese, non-diabetic subjects. These parameters were correlated with in vivo insulin action as measured by the intravenous insulin tolerance test K(itt) values). PC-1 content was negatively correlated with K(itt) values (r = -0.5, p = 0.04) and positively with plasma insulin levels both fasting (r = 0.58, p = 0.009) and after 120 min during oral glucose tolerance test (OGTT) (r = 0.67, p = 0.002). Moreover, adipose tissue PC-1 content: was higher in relatively insulin-resistant subjects (K(itt) values lower than 6) than in relatively insulin-sensitive subjects (K(itt) values higher than 6) (525±49 ng/mg protein vs 33±45, respectively, p = 0.012). Adipose tissue insulin receptor tyrosine kinase activity in response to insulin was significantly lower at all insulin concentrations tested p = 0.017, by two-way analysis of variance test) in insulin-resistant than in insulin-sensitive subjects (K(itt) values lower higher than 6, respectively). In contrast to PC-1, no significant correlation was observed between adipose tissue TNF-α mRNA content and K(itt) values, and plasma insulin levels, both fasting and at after 120 min during OGTT. Also, no difference was observed in TNF-α mRNA content between subjects with K(itt) values higher or lower than 6. These studies in adipose tissue, together with our previous studies in skeletal muscle raise the possibility that PC-1, by regulating insulin receptor function, may play a role in the degree of insulin sensitivity in non-obese, nondiabetic subjects.
AB - In the present study we measured PC-1 content, tumour necrosis factor (TNF)-α gene expression, and insulin stimulation of insulin receptor tyrosine-kinase activity in adipose tissue from nonobese, non-diabetic subjects. These parameters were correlated with in vivo insulin action as measured by the intravenous insulin tolerance test K(itt) values). PC-1 content was negatively correlated with K(itt) values (r = -0.5, p = 0.04) and positively with plasma insulin levels both fasting (r = 0.58, p = 0.009) and after 120 min during oral glucose tolerance test (OGTT) (r = 0.67, p = 0.002). Moreover, adipose tissue PC-1 content: was higher in relatively insulin-resistant subjects (K(itt) values lower than 6) than in relatively insulin-sensitive subjects (K(itt) values higher than 6) (525±49 ng/mg protein vs 33±45, respectively, p = 0.012). Adipose tissue insulin receptor tyrosine kinase activity in response to insulin was significantly lower at all insulin concentrations tested p = 0.017, by two-way analysis of variance test) in insulin-resistant than in insulin-sensitive subjects (K(itt) values lower higher than 6, respectively). In contrast to PC-1, no significant correlation was observed between adipose tissue TNF-α mRNA content and K(itt) values, and plasma insulin levels, both fasting and at after 120 min during OGTT. Also, no difference was observed in TNF-α mRNA content between subjects with K(itt) values higher or lower than 6. These studies in adipose tissue, together with our previous studies in skeletal muscle raise the possibility that PC-1, by regulating insulin receptor function, may play a role in the degree of insulin sensitivity in non-obese, nondiabetic subjects.
KW - Adipose tissue
KW - Insulin receptor tyrosine-kinase inhibitors
KW - Insulin sensitivity
KW - Insulin tolerance test
KW - PC-1
KW - Tumor necrosis factor-α
UR - http://www.scopus.com/inward/record.url?scp=0031026510&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031026510&partnerID=8YFLogxK
U2 - 10.1007/s001250050675
DO - 10.1007/s001250050675
M3 - Article
VL - 40
SP - 282
EP - 289
JO - Diabetologia
JF - Diabetologia
SN - 0012-186X
IS - 3
ER -