Increased adipose tissue PC-1 protein content, but not tumour necrosis factor-α gene expression, is associated with a reduction of both whole body insulin sensitivity and insulin receptor tyrosine-kinase activity

L. Frittitta, J. F. Youngren, P. Sbraccia, M. D'Adamo, A. Buongiorno, R. Vigneri, I. D. Goldfine, V. Trischitta

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

In the present study we measured PC-1 content, tumour necrosis factor (TNF)-α gene expression, and insulin stimulation of insulin receptor tyrosine-kinase activity in adipose tissue from nonobese, non-diabetic subjects. These parameters were correlated with in vivo insulin action as measured by the intravenous insulin tolerance test K(itt) values). PC-1 content was negatively correlated with K(itt) values (r = -0.5, p = 0.04) and positively with plasma insulin levels both fasting (r = 0.58, p = 0.009) and after 120 min during oral glucose tolerance test (OGTT) (r = 0.67, p = 0.002). Moreover, adipose tissue PC-1 content: was higher in relatively insulin-resistant subjects (K(itt) values lower than 6) than in relatively insulin-sensitive subjects (K(itt) values higher than 6) (525±49 ng/mg protein vs 33±45, respectively, p = 0.012). Adipose tissue insulin receptor tyrosine kinase activity in response to insulin was significantly lower at all insulin concentrations tested p = 0.017, by two-way analysis of variance test) in insulin-resistant than in insulin-sensitive subjects (K(itt) values lower higher than 6, respectively). In contrast to PC-1, no significant correlation was observed between adipose tissue TNF-α mRNA content and K(itt) values, and plasma insulin levels, both fasting and at after 120 min during OGTT. Also, no difference was observed in TNF-α mRNA content between subjects with K(itt) values higher or lower than 6. These studies in adipose tissue, together with our previous studies in skeletal muscle raise the possibility that PC-1, by regulating insulin receptor function, may play a role in the degree of insulin sensitivity in non-obese, nondiabetic subjects.

Original languageEnglish
Pages (from-to)282-289
Number of pages8
JournalDiabetologia
Volume40
Issue number3
DOIs
Publication statusPublished - 1997

Fingerprint

Insulin Resistance
Adipose Tissue
Tumor Necrosis Factor-alpha
Insulin
Gene Expression
Proteins
insulin receptor tyrosine kinase
Glucose Tolerance Test
Fasting
Messenger RNA
Insulin Receptor
Analysis of Variance
Skeletal Muscle

Keywords

  • Adipose tissue
  • Insulin receptor tyrosine-kinase inhibitors
  • Insulin sensitivity
  • Insulin tolerance test
  • PC-1
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Increased adipose tissue PC-1 protein content, but not tumour necrosis factor-α gene expression, is associated with a reduction of both whole body insulin sensitivity and insulin receptor tyrosine-kinase activity. / Frittitta, L.; Youngren, J. F.; Sbraccia, P.; D'Adamo, M.; Buongiorno, A.; Vigneri, R.; Goldfine, I. D.; Trischitta, V.

In: Diabetologia, Vol. 40, No. 3, 1997, p. 282-289.

Research output: Contribution to journalArticle

@article{3784662ffe374bc59f9c3132d8433eac,
title = "Increased adipose tissue PC-1 protein content, but not tumour necrosis factor-α gene expression, is associated with a reduction of both whole body insulin sensitivity and insulin receptor tyrosine-kinase activity",
abstract = "In the present study we measured PC-1 content, tumour necrosis factor (TNF)-α gene expression, and insulin stimulation of insulin receptor tyrosine-kinase activity in adipose tissue from nonobese, non-diabetic subjects. These parameters were correlated with in vivo insulin action as measured by the intravenous insulin tolerance test K(itt) values). PC-1 content was negatively correlated with K(itt) values (r = -0.5, p = 0.04) and positively with plasma insulin levels both fasting (r = 0.58, p = 0.009) and after 120 min during oral glucose tolerance test (OGTT) (r = 0.67, p = 0.002). Moreover, adipose tissue PC-1 content: was higher in relatively insulin-resistant subjects (K(itt) values lower than 6) than in relatively insulin-sensitive subjects (K(itt) values higher than 6) (525±49 ng/mg protein vs 33±45, respectively, p = 0.012). Adipose tissue insulin receptor tyrosine kinase activity in response to insulin was significantly lower at all insulin concentrations tested p = 0.017, by two-way analysis of variance test) in insulin-resistant than in insulin-sensitive subjects (K(itt) values lower higher than 6, respectively). In contrast to PC-1, no significant correlation was observed between adipose tissue TNF-α mRNA content and K(itt) values, and plasma insulin levels, both fasting and at after 120 min during OGTT. Also, no difference was observed in TNF-α mRNA content between subjects with K(itt) values higher or lower than 6. These studies in adipose tissue, together with our previous studies in skeletal muscle raise the possibility that PC-1, by regulating insulin receptor function, may play a role in the degree of insulin sensitivity in non-obese, nondiabetic subjects.",
keywords = "Adipose tissue, Insulin receptor tyrosine-kinase inhibitors, Insulin sensitivity, Insulin tolerance test, PC-1, Tumor necrosis factor-α",
author = "L. Frittitta and Youngren, {J. F.} and P. Sbraccia and M. D'Adamo and A. Buongiorno and R. Vigneri and Goldfine, {I. D.} and V. Trischitta",
year = "1997",
doi = "10.1007/s001250050675",
language = "English",
volume = "40",
pages = "282--289",
journal = "Diabetologia",
issn = "0012-186X",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Increased adipose tissue PC-1 protein content, but not tumour necrosis factor-α gene expression, is associated with a reduction of both whole body insulin sensitivity and insulin receptor tyrosine-kinase activity

AU - Frittitta, L.

AU - Youngren, J. F.

AU - Sbraccia, P.

AU - D'Adamo, M.

AU - Buongiorno, A.

AU - Vigneri, R.

AU - Goldfine, I. D.

AU - Trischitta, V.

PY - 1997

Y1 - 1997

N2 - In the present study we measured PC-1 content, tumour necrosis factor (TNF)-α gene expression, and insulin stimulation of insulin receptor tyrosine-kinase activity in adipose tissue from nonobese, non-diabetic subjects. These parameters were correlated with in vivo insulin action as measured by the intravenous insulin tolerance test K(itt) values). PC-1 content was negatively correlated with K(itt) values (r = -0.5, p = 0.04) and positively with plasma insulin levels both fasting (r = 0.58, p = 0.009) and after 120 min during oral glucose tolerance test (OGTT) (r = 0.67, p = 0.002). Moreover, adipose tissue PC-1 content: was higher in relatively insulin-resistant subjects (K(itt) values lower than 6) than in relatively insulin-sensitive subjects (K(itt) values higher than 6) (525±49 ng/mg protein vs 33±45, respectively, p = 0.012). Adipose tissue insulin receptor tyrosine kinase activity in response to insulin was significantly lower at all insulin concentrations tested p = 0.017, by two-way analysis of variance test) in insulin-resistant than in insulin-sensitive subjects (K(itt) values lower higher than 6, respectively). In contrast to PC-1, no significant correlation was observed between adipose tissue TNF-α mRNA content and K(itt) values, and plasma insulin levels, both fasting and at after 120 min during OGTT. Also, no difference was observed in TNF-α mRNA content between subjects with K(itt) values higher or lower than 6. These studies in adipose tissue, together with our previous studies in skeletal muscle raise the possibility that PC-1, by regulating insulin receptor function, may play a role in the degree of insulin sensitivity in non-obese, nondiabetic subjects.

AB - In the present study we measured PC-1 content, tumour necrosis factor (TNF)-α gene expression, and insulin stimulation of insulin receptor tyrosine-kinase activity in adipose tissue from nonobese, non-diabetic subjects. These parameters were correlated with in vivo insulin action as measured by the intravenous insulin tolerance test K(itt) values). PC-1 content was negatively correlated with K(itt) values (r = -0.5, p = 0.04) and positively with plasma insulin levels both fasting (r = 0.58, p = 0.009) and after 120 min during oral glucose tolerance test (OGTT) (r = 0.67, p = 0.002). Moreover, adipose tissue PC-1 content: was higher in relatively insulin-resistant subjects (K(itt) values lower than 6) than in relatively insulin-sensitive subjects (K(itt) values higher than 6) (525±49 ng/mg protein vs 33±45, respectively, p = 0.012). Adipose tissue insulin receptor tyrosine kinase activity in response to insulin was significantly lower at all insulin concentrations tested p = 0.017, by two-way analysis of variance test) in insulin-resistant than in insulin-sensitive subjects (K(itt) values lower higher than 6, respectively). In contrast to PC-1, no significant correlation was observed between adipose tissue TNF-α mRNA content and K(itt) values, and plasma insulin levels, both fasting and at after 120 min during OGTT. Also, no difference was observed in TNF-α mRNA content between subjects with K(itt) values higher or lower than 6. These studies in adipose tissue, together with our previous studies in skeletal muscle raise the possibility that PC-1, by regulating insulin receptor function, may play a role in the degree of insulin sensitivity in non-obese, nondiabetic subjects.

KW - Adipose tissue

KW - Insulin receptor tyrosine-kinase inhibitors

KW - Insulin sensitivity

KW - Insulin tolerance test

KW - PC-1

KW - Tumor necrosis factor-α

UR - http://www.scopus.com/inward/record.url?scp=0031026510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031026510&partnerID=8YFLogxK

U2 - 10.1007/s001250050675

DO - 10.1007/s001250050675

M3 - Article

VL - 40

SP - 282

EP - 289

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 3

ER -