TY - JOUR
T1 - Increased c-myc oncogene expression in Ewing's sarcoma
T2 - Correlation with Ki67 proliferation index
AU - Sollazzo, Maria Rosa
AU - Benassi, Maria Serena
AU - Magagnoli, Giovanna
AU - Gamberi, Gabriella
AU - Molendini, Lara
AU - Ragazzini, Paola
AU - Merli, Mara
AU - Ferrari, Cristina
AU - Balladelli, Alba
AU - Picci, Piero
PY - 1999/5
Y1 - 1999/5
N2 - Aims and background: Ewing's sarcoma is a highly malignant musculoskeletal tumor composed of small round cells. Although important results have been achieved with surgery associated with chemotherapy, recurrent disease is still a major problem. In order to define new prognostic factors useful for therapeutic decision-making, we conducted a study on 38 Ewing's sarcoma samples in which c-myc oncogene expression and Ki67 proliferation index were correlated with clinical outcome. Methods and study design: Nineteen patients developed metastases during follow-up and 10 of these patients died. C-myc and Ki67 protein expression was evaluated by immunohistochemistry performed on 5 μm formalin-fixed and paraffin-embedded sections, while the c-myc mRNA transcript was localized using in situ hybridization. Results: A statistically positive correlation was found between c-myc protein and Ki67 (P = 0.001) and c-myc mRNA and Ki67 expression (P = 0.047). The 38 patients were divided into two groups using as the cutoff 50% of Ki67-positive cells. The disease-free survival and overall survival estimates were 68% and 90%, respectively, in the group of patients with a percentage of Ki67-positive cells
AB - Aims and background: Ewing's sarcoma is a highly malignant musculoskeletal tumor composed of small round cells. Although important results have been achieved with surgery associated with chemotherapy, recurrent disease is still a major problem. In order to define new prognostic factors useful for therapeutic decision-making, we conducted a study on 38 Ewing's sarcoma samples in which c-myc oncogene expression and Ki67 proliferation index were correlated with clinical outcome. Methods and study design: Nineteen patients developed metastases during follow-up and 10 of these patients died. C-myc and Ki67 protein expression was evaluated by immunohistochemistry performed on 5 μm formalin-fixed and paraffin-embedded sections, while the c-myc mRNA transcript was localized using in situ hybridization. Results: A statistically positive correlation was found between c-myc protein and Ki67 (P = 0.001) and c-myc mRNA and Ki67 expression (P = 0.047). The 38 patients were divided into two groups using as the cutoff 50% of Ki67-positive cells. The disease-free survival and overall survival estimates were 68% and 90%, respectively, in the group of patients with a percentage of Ki67-positive cells
KW - c-myc
KW - Ewing's sarcoma
KW - Ki67
UR - http://www.scopus.com/inward/record.url?scp=0032807399&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032807399&partnerID=8YFLogxK
M3 - Article
C2 - 10426126
AN - SCOPUS:0032807399
VL - 85
SP - 167
EP - 173
JO - Tumori
JF - Tumori
SN - 0300-8916
IS - 3
ER -