Increased detection of antibody to hepatitis C virus in renal transplant patients by third-generation assays

Roberta Soffredini, Mariagrazia Rumi, Pietro Lampertico, Adriana Aroldi, Antonio Tarantino, Claudio Ponticelli, Massimo Colombo

Research output: Contribution to journalArticlepeer-review


To assess the sensitivity and specificity of third-generation assays for antibody to hepatitis C virus (anti-HCV), sera from 244 renal transplant patients (113 positive for anti-HCV enzyme-linked immunosorbent assay [EMSA]- 2) were studied. Hepatitis C virus RNA was detected by a reverse-transcripted nested polymerase chain reaction. Antibody to HCV was detected by ELISA-3 in 108 (96%) ELISA-2-positive samples. Five (4%) ELISA-2-positive sera were negative by both ELISA-3 and polymerase chain reaction. In the anti-HCV- negative group, six (5%) additional cases were ELISA-3-positive; three of these were confirmed by recombinant immunoblot assay-3 (RIBA-3) and polymerase chain reaction. Recombinant immunoblot assay-3 was used to resolve 82 RIBA-2-indeterminate and three RIBA-2-negative sera. Using RIBA-3, 49 (60%) RIBA-2-indeterminate samples were positive, five (6%) ELISA-3- negative samples were negative, and 28 (34%) were remained indeterminate. Recombinant immunoblot assay-2-negative samples were indeterminate with RIBA-3. Hepatitis C virus RNA was detected in all RIBA-3-positive and 58% of the RIBA-3- indeterminate samples. Third-generation assays for anti-HCV are more sensitive and specific than second-generation assays in renal transplant patients.

Original languageEnglish
Pages (from-to)437-440
Number of pages4
JournalAmerican Journal of Kidney Diseases
Issue number3
Publication statusPublished - Sep 1996


  • antibody to hepatitis C virus
  • Hepatitis C virus
  • hepatitis C virus RNA
  • recombinant immunoblot assay
  • renal transplantation
  • third-generation tests

ASJC Scopus subject areas

  • Nephrology


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