Increased expression of α(1,3)-fucosyltransferase-VII and P-selectin binding of synovial fluid T cells in juvenile idiopathic arthritis

Fabrizio De Benedetti, Patrizia Pignatti, Mauro Biffi, Elisa Bono, Smaira Wahid, Francesca Ingegnoli, Sheng yung P Chang, Heather Alexander, Margherita Massa, Angela Pistorio, Alberto Martini, Costantino Pitzalis, Francesco Sinigaglia, Lars Rogge

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Abstract

Objective. The mechanisms controlling the recruitment of T helper type 1 (Th1) cells to the inflamed synovium are not fully understood. Here, we focus on α(1,3)-fucosyltransferase-VII (FucT-VII), an enzyme responsible for the generation of functional P- and E-selectin ligands that is upregulated in Th1 cells. Methods. Expression of transcripts encoding FucT-VII, interferon-γ (IFN-γ), and interleukin 12Rβ2 (IL-12Rβ2) were analyzed in T cells purified from synovial fluid (SF) and from peripheral blood (PB) of children with juvenile idiopathic arthritis (JIA) using kinetic reverse transcriptase polymerase chain reaction analysis. Binding of SF and PB T cells to P-selectin was determined by flow cytometry using a soluble P-selectin/IgG1 fusion molecule. Recruitment of T cells to synovial tissue in vivo was studied by analyzing the migration of FucT-VII transfected Jurkat T cells into human rheumatoid synovial tissue grafted into SCID mice. Results. In patients with JIA, the mRNA levels of FucT-VII, as well as of IFN-γ and IL-12Rβ2, were up-regulated in SF T cells compared to paired PB T cells. A higher expression of FucT-VII mRNA in SF T cells was associated with increased binding of T cells to P-selectin. Moreover, FucT-VII expression and increased P-selectin binding capacity of T cells were associated with a polyarticular course of oligoarticular JIA. Expression of FucT-VII in Jurkat T cells resulted in an increased accumulation of these cells in human rheumatoid synovial tissue grafted into SCID mice. Conclusion. Our data indicate an important role of FucT-VII in the enhanced homing of T cells to the inflamed synovium.

Original languageEnglish
Pages (from-to)1611-1615
Number of pages5
JournalJournal of Rheumatology
Volume30
Issue number7
Publication statusPublished - Jul 1 2003

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galactoside 3-fucosyltransferase
P-Selectin
Juvenile Arthritis
Synovial Fluid
Fucosyltransferases
T-Lymphocytes
Th1 Cells
Jurkat Cells
SCID Mice
Synovial Membrane
Interleukins
Interferons
Blood Cells

Keywords

  • Adhesion molecules
  • Cell trafficking
  • Inflammation
  • Rheumatoid arthritis
  • T lymphocytes
  • Th1/Th2 cells

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Increased expression of α(1,3)-fucosyltransferase-VII and P-selectin binding of synovial fluid T cells in juvenile idiopathic arthritis. / De Benedetti, Fabrizio; Pignatti, Patrizia; Biffi, Mauro; Bono, Elisa; Wahid, Smaira; Ingegnoli, Francesca; Chang, Sheng yung P; Alexander, Heather; Massa, Margherita; Pistorio, Angela; Martini, Alberto; Pitzalis, Costantino; Sinigaglia, Francesco; Rogge, Lars.

In: Journal of Rheumatology, Vol. 30, No. 7, 01.07.2003, p. 1611-1615.

Research output: Contribution to journalArticle

De Benedetti, F, Pignatti, P, Biffi, M, Bono, E, Wahid, S, Ingegnoli, F, Chang, SYP, Alexander, H, Massa, M, Pistorio, A, Martini, A, Pitzalis, C, Sinigaglia, F & Rogge, L 2003, 'Increased expression of α(1,3)-fucosyltransferase-VII and P-selectin binding of synovial fluid T cells in juvenile idiopathic arthritis', Journal of Rheumatology, vol. 30, no. 7, pp. 1611-1615.
De Benedetti, Fabrizio ; Pignatti, Patrizia ; Biffi, Mauro ; Bono, Elisa ; Wahid, Smaira ; Ingegnoli, Francesca ; Chang, Sheng yung P ; Alexander, Heather ; Massa, Margherita ; Pistorio, Angela ; Martini, Alberto ; Pitzalis, Costantino ; Sinigaglia, Francesco ; Rogge, Lars. / Increased expression of α(1,3)-fucosyltransferase-VII and P-selectin binding of synovial fluid T cells in juvenile idiopathic arthritis. In: Journal of Rheumatology. 2003 ; Vol. 30, No. 7. pp. 1611-1615.
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abstract = "Objective. The mechanisms controlling the recruitment of T helper type 1 (Th1) cells to the inflamed synovium are not fully understood. Here, we focus on α(1,3)-fucosyltransferase-VII (FucT-VII), an enzyme responsible for the generation of functional P- and E-selectin ligands that is upregulated in Th1 cells. Methods. Expression of transcripts encoding FucT-VII, interferon-γ (IFN-γ), and interleukin 12Rβ2 (IL-12Rβ2) were analyzed in T cells purified from synovial fluid (SF) and from peripheral blood (PB) of children with juvenile idiopathic arthritis (JIA) using kinetic reverse transcriptase polymerase chain reaction analysis. Binding of SF and PB T cells to P-selectin was determined by flow cytometry using a soluble P-selectin/IgG1 fusion molecule. Recruitment of T cells to synovial tissue in vivo was studied by analyzing the migration of FucT-VII transfected Jurkat T cells into human rheumatoid synovial tissue grafted into SCID mice. Results. In patients with JIA, the mRNA levels of FucT-VII, as well as of IFN-γ and IL-12Rβ2, were up-regulated in SF T cells compared to paired PB T cells. A higher expression of FucT-VII mRNA in SF T cells was associated with increased binding of T cells to P-selectin. Moreover, FucT-VII expression and increased P-selectin binding capacity of T cells were associated with a polyarticular course of oligoarticular JIA. Expression of FucT-VII in Jurkat T cells resulted in an increased accumulation of these cells in human rheumatoid synovial tissue grafted into SCID mice. Conclusion. Our data indicate an important role of FucT-VII in the enhanced homing of T cells to the inflamed synovium.",
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T1 - Increased expression of α(1,3)-fucosyltransferase-VII and P-selectin binding of synovial fluid T cells in juvenile idiopathic arthritis

AU - De Benedetti, Fabrizio

AU - Pignatti, Patrizia

AU - Biffi, Mauro

AU - Bono, Elisa

AU - Wahid, Smaira

AU - Ingegnoli, Francesca

AU - Chang, Sheng yung P

AU - Alexander, Heather

AU - Massa, Margherita

AU - Pistorio, Angela

AU - Martini, Alberto

AU - Pitzalis, Costantino

AU - Sinigaglia, Francesco

AU - Rogge, Lars

PY - 2003/7/1

Y1 - 2003/7/1

N2 - Objective. The mechanisms controlling the recruitment of T helper type 1 (Th1) cells to the inflamed synovium are not fully understood. Here, we focus on α(1,3)-fucosyltransferase-VII (FucT-VII), an enzyme responsible for the generation of functional P- and E-selectin ligands that is upregulated in Th1 cells. Methods. Expression of transcripts encoding FucT-VII, interferon-γ (IFN-γ), and interleukin 12Rβ2 (IL-12Rβ2) were analyzed in T cells purified from synovial fluid (SF) and from peripheral blood (PB) of children with juvenile idiopathic arthritis (JIA) using kinetic reverse transcriptase polymerase chain reaction analysis. Binding of SF and PB T cells to P-selectin was determined by flow cytometry using a soluble P-selectin/IgG1 fusion molecule. Recruitment of T cells to synovial tissue in vivo was studied by analyzing the migration of FucT-VII transfected Jurkat T cells into human rheumatoid synovial tissue grafted into SCID mice. Results. In patients with JIA, the mRNA levels of FucT-VII, as well as of IFN-γ and IL-12Rβ2, were up-regulated in SF T cells compared to paired PB T cells. A higher expression of FucT-VII mRNA in SF T cells was associated with increased binding of T cells to P-selectin. Moreover, FucT-VII expression and increased P-selectin binding capacity of T cells were associated with a polyarticular course of oligoarticular JIA. Expression of FucT-VII in Jurkat T cells resulted in an increased accumulation of these cells in human rheumatoid synovial tissue grafted into SCID mice. Conclusion. Our data indicate an important role of FucT-VII in the enhanced homing of T cells to the inflamed synovium.

AB - Objective. The mechanisms controlling the recruitment of T helper type 1 (Th1) cells to the inflamed synovium are not fully understood. Here, we focus on α(1,3)-fucosyltransferase-VII (FucT-VII), an enzyme responsible for the generation of functional P- and E-selectin ligands that is upregulated in Th1 cells. Methods. Expression of transcripts encoding FucT-VII, interferon-γ (IFN-γ), and interleukin 12Rβ2 (IL-12Rβ2) were analyzed in T cells purified from synovial fluid (SF) and from peripheral blood (PB) of children with juvenile idiopathic arthritis (JIA) using kinetic reverse transcriptase polymerase chain reaction analysis. Binding of SF and PB T cells to P-selectin was determined by flow cytometry using a soluble P-selectin/IgG1 fusion molecule. Recruitment of T cells to synovial tissue in vivo was studied by analyzing the migration of FucT-VII transfected Jurkat T cells into human rheumatoid synovial tissue grafted into SCID mice. Results. In patients with JIA, the mRNA levels of FucT-VII, as well as of IFN-γ and IL-12Rβ2, were up-regulated in SF T cells compared to paired PB T cells. A higher expression of FucT-VII mRNA in SF T cells was associated with increased binding of T cells to P-selectin. Moreover, FucT-VII expression and increased P-selectin binding capacity of T cells were associated with a polyarticular course of oligoarticular JIA. Expression of FucT-VII in Jurkat T cells resulted in an increased accumulation of these cells in human rheumatoid synovial tissue grafted into SCID mice. Conclusion. Our data indicate an important role of FucT-VII in the enhanced homing of T cells to the inflamed synovium.

KW - Adhesion molecules

KW - Cell trafficking

KW - Inflammation

KW - Rheumatoid arthritis

KW - T lymphocytes

KW - Th1/Th2 cells

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