Increased expression of interleukin-22 in patients with giant cell arteritis

Alessandro Zerbini; Francesco Muratore; Luigi Boiardi; Francesco Ciccia; Martina Bonacini; Lucia Belloni; Alberto Cavazza; Luca Cimino; Antonio Moramarco; Riccardo Alessandro; Aroldo Rizzo; Maria Parmeggiani; Carlo Salvarani; Stefania Croci

doi:10.1093/rheumatology/kex334

Increased expression of interleukin-22 in patients with giant cell arteritis

Alessandro Zerbini, Francesco Muratore, Luigi Boiardi, Francesco Ciccia, Martina Bonacini, Lucia Belloni, Alberto Cavazza, Luca Cimino, Antonio Moramarco, Riccardo Alessandro, Aroldo Rizzo, Maria Parmeggiani, Carlo Salvarani, Stefania Croci

Arcispedale Santa Maria Nuova

Research output: Contribution to journal › Article › peer-review

Abstract

Objectives: GCA is characterized by arterial remodelling driven by inflammation. IL-22 is an attractive cytokine which acts at the crosstalk between immune and stromal cells. We hypothesized that IL-22 might be induced in GCA and might be involved in disease pathogenesis.

Methods: Patients subjected to temporal artery biopsies (TABs) naïve from therapy were enrolled: 27 biopsy-proven GCA, 8 biopsy-negative GCA, 21 biopsy-negative non-GCA patients. Expression of IL-22 was determined in TABs by immunohystochemistry, in plasma by ELISA, in peripheral blood mononuclear cells by real-time PCR and flow cytometry. Effects of IL-22 on viability and gene expression of primary cultures obtained from TABs were also evaluated.

Results: Inflamed TABs from GCA patients showed a higher expression of IL-22 and IL-22 specific receptor subunit (IL-22R1) than non-inflamed TABs. IL-22 was expressed in infiltrating immune cells and spindle shaped cells, IL-22R1 was expressed in endothelial cells. Patients with biopsy-proven GCA showed increased levels of IL-22 in plasma than patients with biopsy-negative GCA, without GCA and healthy subjects. Peripheral blood mononuclear cells from GCA patients expressed higher IL-22 transcript than healthy subjects. After stimulation in vitro with phorbol 12-myristate 13-acetate and ionomycin, the frequencies of Th22 and IL-22+ CD4+ lymphocytes were similar between patients with and without GCA. Treatment with IL-22 of primary cultures obtained from TABs increased cell viability under stress conditions and expression of B-cell activating factor.

Conclusion: IL-22 is increased in patients with GCA and affects viability and gene expression of arterial cells, supporting a potential role in disease pathogenesis.

Original language	English
Pages (from-to)	64-72
Number of pages	9
Journal	Rheumatology (Oxford, England)
Volume	57
Issue number	1
DOIs	https://doi.org/10.1093/rheumatology/kex334
Publication status	Published - Jan 1 2018

Keywords

arterial remodelling
autoimmunity
giant cell arteritis
inflammation
interleukin-22
pathogenesis

ASJC Scopus subject areas

Rheumatology
Pharmacology (medical)

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Increased expression of interleukin-22 in patients with giant cell arteritis. / Zerbini, Alessandro; Muratore, Francesco; Boiardi, Luigi; Ciccia, Francesco; Bonacini, Martina; Belloni, Lucia; Cavazza, Alberto ; Cimino, Luca; Moramarco, Antonio; Alessandro, Riccardo; Rizzo, Aroldo; Parmeggiani, Maria; Salvarani, Carlo ; Croci, Stefania.

In: Rheumatology (Oxford, England), Vol. 57, No. 1, 01.01.2018, p. 64-72.

Research output: Contribution to journal › Article › peer-review

Zerbini, Alessandro ; Muratore, Francesco ; Boiardi, Luigi ; Ciccia, Francesco ; Bonacini, Martina ; Belloni, Lucia ; Cavazza, Alberto ; Cimino, Luca ; Moramarco, Antonio ; Alessandro, Riccardo ; Rizzo, Aroldo ; Parmeggiani, Maria ; Salvarani, Carlo ; Croci, Stefania. / Increased expression of interleukin-22 in patients with giant cell arteritis. In: Rheumatology (Oxford, England). 2018 ; Vol. 57, No. 1. pp. 64-72.

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abstract = "Objectives: GCA is characterized by arterial remodelling driven by inflammation. IL-22 is an attractive cytokine which acts at the crosstalk between immune and stromal cells. We hypothesized that IL-22 might be induced in GCA and might be involved in disease pathogenesis.Methods: Patients subjected to temporal artery biopsies (TABs) na{\"i}ve from therapy were enrolled: 27 biopsy-proven GCA, 8 biopsy-negative GCA, 21 biopsy-negative non-GCA patients. Expression of IL-22 was determined in TABs by immunohystochemistry, in plasma by ELISA, in peripheral blood mononuclear cells by real-time PCR and flow cytometry. Effects of IL-22 on viability and gene expression of primary cultures obtained from TABs were also evaluated.Results: Inflamed TABs from GCA patients showed a higher expression of IL-22 and IL-22 specific receptor subunit (IL-22R1) than non-inflamed TABs. IL-22 was expressed in infiltrating immune cells and spindle shaped cells, IL-22R1 was expressed in endothelial cells. Patients with biopsy-proven GCA showed increased levels of IL-22 in plasma than patients with biopsy-negative GCA, without GCA and healthy subjects. Peripheral blood mononuclear cells from GCA patients expressed higher IL-22 transcript than healthy subjects. After stimulation in vitro with phorbol 12-myristate 13-acetate and ionomycin, the frequencies of Th22 and IL-22+ CD4+ lymphocytes were similar between patients with and without GCA. Treatment with IL-22 of primary cultures obtained from TABs increased cell viability under stress conditions and expression of B-cell activating factor.Conclusion: IL-22 is increased in patients with GCA and affects viability and gene expression of arterial cells, supporting a potential role in disease pathogenesis.",

keywords = "arterial remodelling, autoimmunity, giant cell arteritis, inflammation, interleukin-22, pathogenesis",

author = "Alessandro Zerbini and Francesco Muratore and Luigi Boiardi and Francesco Ciccia and Martina Bonacini and Lucia Belloni and Alberto Cavazza and Luca Cimino and Antonio Moramarco and Riccardo Alessandro and Aroldo Rizzo and Maria Parmeggiani and Carlo Salvarani and Stefania Croci",

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T1 - Increased expression of interleukin-22 in patients with giant cell arteritis

AU - Zerbini, Alessandro

AU - Muratore, Francesco

AU - Boiardi, Luigi

AU - Ciccia, Francesco

AU - Bonacini, Martina

AU - Belloni, Lucia

AU - Cavazza, Alberto

AU - Cimino, Luca

AU - Moramarco, Antonio

AU - Alessandro, Riccardo

AU - Rizzo, Aroldo

AU - Parmeggiani, Maria

AU - Salvarani, Carlo

AU - Croci, Stefania

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Objectives: GCA is characterized by arterial remodelling driven by inflammation. IL-22 is an attractive cytokine which acts at the crosstalk between immune and stromal cells. We hypothesized that IL-22 might be induced in GCA and might be involved in disease pathogenesis.Methods: Patients subjected to temporal artery biopsies (TABs) naïve from therapy were enrolled: 27 biopsy-proven GCA, 8 biopsy-negative GCA, 21 biopsy-negative non-GCA patients. Expression of IL-22 was determined in TABs by immunohystochemistry, in plasma by ELISA, in peripheral blood mononuclear cells by real-time PCR and flow cytometry. Effects of IL-22 on viability and gene expression of primary cultures obtained from TABs were also evaluated.Results: Inflamed TABs from GCA patients showed a higher expression of IL-22 and IL-22 specific receptor subunit (IL-22R1) than non-inflamed TABs. IL-22 was expressed in infiltrating immune cells and spindle shaped cells, IL-22R1 was expressed in endothelial cells. Patients with biopsy-proven GCA showed increased levels of IL-22 in plasma than patients with biopsy-negative GCA, without GCA and healthy subjects. Peripheral blood mononuclear cells from GCA patients expressed higher IL-22 transcript than healthy subjects. After stimulation in vitro with phorbol 12-myristate 13-acetate and ionomycin, the frequencies of Th22 and IL-22+ CD4+ lymphocytes were similar between patients with and without GCA. Treatment with IL-22 of primary cultures obtained from TABs increased cell viability under stress conditions and expression of B-cell activating factor.Conclusion: IL-22 is increased in patients with GCA and affects viability and gene expression of arterial cells, supporting a potential role in disease pathogenesis.

AB - Objectives: GCA is characterized by arterial remodelling driven by inflammation. IL-22 is an attractive cytokine which acts at the crosstalk between immune and stromal cells. We hypothesized that IL-22 might be induced in GCA and might be involved in disease pathogenesis.Methods: Patients subjected to temporal artery biopsies (TABs) naïve from therapy were enrolled: 27 biopsy-proven GCA, 8 biopsy-negative GCA, 21 biopsy-negative non-GCA patients. Expression of IL-22 was determined in TABs by immunohystochemistry, in plasma by ELISA, in peripheral blood mononuclear cells by real-time PCR and flow cytometry. Effects of IL-22 on viability and gene expression of primary cultures obtained from TABs were also evaluated.Results: Inflamed TABs from GCA patients showed a higher expression of IL-22 and IL-22 specific receptor subunit (IL-22R1) than non-inflamed TABs. IL-22 was expressed in infiltrating immune cells and spindle shaped cells, IL-22R1 was expressed in endothelial cells. Patients with biopsy-proven GCA showed increased levels of IL-22 in plasma than patients with biopsy-negative GCA, without GCA and healthy subjects. Peripheral blood mononuclear cells from GCA patients expressed higher IL-22 transcript than healthy subjects. After stimulation in vitro with phorbol 12-myristate 13-acetate and ionomycin, the frequencies of Th22 and IL-22+ CD4+ lymphocytes were similar between patients with and without GCA. Treatment with IL-22 of primary cultures obtained from TABs increased cell viability under stress conditions and expression of B-cell activating factor.Conclusion: IL-22 is increased in patients with GCA and affects viability and gene expression of arterial cells, supporting a potential role in disease pathogenesis.

KW - arterial remodelling

KW - autoimmunity

KW - giant cell arteritis

KW - inflammation

KW - interleukin-22

KW - pathogenesis

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