Increased expression of myeloid antigen markers in adult acute lymphoblastic leukaemia patients: Diagnostic and prognostic implications

F. Lauria, D. Raspadori, G. Martinelli, D. Rondelli, M. A. Ventura, P. Farabegoli, P. Tosi, N. Testoni, G. Visani, A. Zaccaria, B. Gamberi, A. Cenacchi, S. Tura

Research output: Contribution to journalArticle

Abstract

By applying direct immunofluorescence with a dual-staining technique we were able to demonstrate that 20/37 (54%) patients with acute lymphoblastic leukaemia (ALL) expressed both lymphoid and myeloid antigens on the same leukaemic cells. CD13 and CD33 myeloid antigens were detected in 18/20 and in 15/20 cases respectively, and both in 13. Molecular studies confirmed that the four patients with T-cell phenotype had molecular rearrangement of T-cell receptor (TcR) β chain, and 26 ALL patients with 'B-cell' phenotype showed JH rearrangement. Two ALL patients without (ALL/My-) and three with myeloid antigens (ALL/My+) also demonstrated bcr/abl rearrangement. Both groups of patients had similar presenting features such as age, sex, Hb level, white blood cells, platelet counts and cytogenetic features. Complete response was achieved in 16/17 (94%) ALL/My- patients and in 15/18 (83%) ALL/My+ patients (two deaths occurred during induction) with a mean duration of 17 and 16 months respectively and with similar survival and event-free survival curves. Myeloid antigen expression in adult ALL patients may occur more frequently than previously reported. The presence of myeloid antigen does not identify, in our series, a higher-risk subgroup of patients, although lack of any statistical evidence of prognostic significance needs to be confirmed in a larger case study.

Original languageEnglish
Pages (from-to)286-292
Number of pages7
JournalBritish Journal of Haematology
Volume87
Issue number2
Publication statusPublished - 1994

Fingerprint

Differentiation Antigens
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Antigens
Sialic Acid Binding Ig-like Lectin 3
Phenotype
Direct Fluorescent Antibody Technique
T-Cell Antigen Receptor
Platelet Count
Leukocyte Count
Cytogenetics
Disease-Free Survival
B-Lymphocytes
Staining and Labeling
T-Lymphocytes
Survival

Keywords

  • Acute lymphoblastic leukaemia
  • Immunofluorescence
  • Myeloid antigen markers

ASJC Scopus subject areas

  • Hematology

Cite this

Lauria, F., Raspadori, D., Martinelli, G., Rondelli, D., Ventura, M. A., Farabegoli, P., ... Tura, S. (1994). Increased expression of myeloid antigen markers in adult acute lymphoblastic leukaemia patients: Diagnostic and prognostic implications. British Journal of Haematology, 87(2), 286-292.

Increased expression of myeloid antigen markers in adult acute lymphoblastic leukaemia patients : Diagnostic and prognostic implications. / Lauria, F.; Raspadori, D.; Martinelli, G.; Rondelli, D.; Ventura, M. A.; Farabegoli, P.; Tosi, P.; Testoni, N.; Visani, G.; Zaccaria, A.; Gamberi, B.; Cenacchi, A.; Tura, S.

In: British Journal of Haematology, Vol. 87, No. 2, 1994, p. 286-292.

Research output: Contribution to journalArticle

Lauria, F, Raspadori, D, Martinelli, G, Rondelli, D, Ventura, MA, Farabegoli, P, Tosi, P, Testoni, N, Visani, G, Zaccaria, A, Gamberi, B, Cenacchi, A & Tura, S 1994, 'Increased expression of myeloid antigen markers in adult acute lymphoblastic leukaemia patients: Diagnostic and prognostic implications', British Journal of Haematology, vol. 87, no. 2, pp. 286-292.
Lauria, F. ; Raspadori, D. ; Martinelli, G. ; Rondelli, D. ; Ventura, M. A. ; Farabegoli, P. ; Tosi, P. ; Testoni, N. ; Visani, G. ; Zaccaria, A. ; Gamberi, B. ; Cenacchi, A. ; Tura, S. / Increased expression of myeloid antigen markers in adult acute lymphoblastic leukaemia patients : Diagnostic and prognostic implications. In: British Journal of Haematology. 1994 ; Vol. 87, No. 2. pp. 286-292.
@article{cefd7deb8199439887abda97f3bf30ce,
title = "Increased expression of myeloid antigen markers in adult acute lymphoblastic leukaemia patients: Diagnostic and prognostic implications",
abstract = "By applying direct immunofluorescence with a dual-staining technique we were able to demonstrate that 20/37 (54{\%}) patients with acute lymphoblastic leukaemia (ALL) expressed both lymphoid and myeloid antigens on the same leukaemic cells. CD13 and CD33 myeloid antigens were detected in 18/20 and in 15/20 cases respectively, and both in 13. Molecular studies confirmed that the four patients with T-cell phenotype had molecular rearrangement of T-cell receptor (TcR) β chain, and 26 ALL patients with 'B-cell' phenotype showed JH rearrangement. Two ALL patients without (ALL/My-) and three with myeloid antigens (ALL/My+) also demonstrated bcr/abl rearrangement. Both groups of patients had similar presenting features such as age, sex, Hb level, white blood cells, platelet counts and cytogenetic features. Complete response was achieved in 16/17 (94{\%}) ALL/My- patients and in 15/18 (83{\%}) ALL/My+ patients (two deaths occurred during induction) with a mean duration of 17 and 16 months respectively and with similar survival and event-free survival curves. Myeloid antigen expression in adult ALL patients may occur more frequently than previously reported. The presence of myeloid antigen does not identify, in our series, a higher-risk subgroup of patients, although lack of any statistical evidence of prognostic significance needs to be confirmed in a larger case study.",
keywords = "Acute lymphoblastic leukaemia, Immunofluorescence, Myeloid antigen markers",
author = "F. Lauria and D. Raspadori and G. Martinelli and D. Rondelli and Ventura, {M. A.} and P. Farabegoli and P. Tosi and N. Testoni and G. Visani and A. Zaccaria and B. Gamberi and A. Cenacchi and S. Tura",
year = "1994",
language = "English",
volume = "87",
pages = "286--292",
journal = "British Journal of Haematology",
issn = "0007-1048",
publisher = "John Wiley & Sons, Ltd (10.1111)",
number = "2",

}

TY - JOUR

T1 - Increased expression of myeloid antigen markers in adult acute lymphoblastic leukaemia patients

T2 - Diagnostic and prognostic implications

AU - Lauria, F.

AU - Raspadori, D.

AU - Martinelli, G.

AU - Rondelli, D.

AU - Ventura, M. A.

AU - Farabegoli, P.

AU - Tosi, P.

AU - Testoni, N.

AU - Visani, G.

AU - Zaccaria, A.

AU - Gamberi, B.

AU - Cenacchi, A.

AU - Tura, S.

PY - 1994

Y1 - 1994

N2 - By applying direct immunofluorescence with a dual-staining technique we were able to demonstrate that 20/37 (54%) patients with acute lymphoblastic leukaemia (ALL) expressed both lymphoid and myeloid antigens on the same leukaemic cells. CD13 and CD33 myeloid antigens were detected in 18/20 and in 15/20 cases respectively, and both in 13. Molecular studies confirmed that the four patients with T-cell phenotype had molecular rearrangement of T-cell receptor (TcR) β chain, and 26 ALL patients with 'B-cell' phenotype showed JH rearrangement. Two ALL patients without (ALL/My-) and three with myeloid antigens (ALL/My+) also demonstrated bcr/abl rearrangement. Both groups of patients had similar presenting features such as age, sex, Hb level, white blood cells, platelet counts and cytogenetic features. Complete response was achieved in 16/17 (94%) ALL/My- patients and in 15/18 (83%) ALL/My+ patients (two deaths occurred during induction) with a mean duration of 17 and 16 months respectively and with similar survival and event-free survival curves. Myeloid antigen expression in adult ALL patients may occur more frequently than previously reported. The presence of myeloid antigen does not identify, in our series, a higher-risk subgroup of patients, although lack of any statistical evidence of prognostic significance needs to be confirmed in a larger case study.

AB - By applying direct immunofluorescence with a dual-staining technique we were able to demonstrate that 20/37 (54%) patients with acute lymphoblastic leukaemia (ALL) expressed both lymphoid and myeloid antigens on the same leukaemic cells. CD13 and CD33 myeloid antigens were detected in 18/20 and in 15/20 cases respectively, and both in 13. Molecular studies confirmed that the four patients with T-cell phenotype had molecular rearrangement of T-cell receptor (TcR) β chain, and 26 ALL patients with 'B-cell' phenotype showed JH rearrangement. Two ALL patients without (ALL/My-) and three with myeloid antigens (ALL/My+) also demonstrated bcr/abl rearrangement. Both groups of patients had similar presenting features such as age, sex, Hb level, white blood cells, platelet counts and cytogenetic features. Complete response was achieved in 16/17 (94%) ALL/My- patients and in 15/18 (83%) ALL/My+ patients (two deaths occurred during induction) with a mean duration of 17 and 16 months respectively and with similar survival and event-free survival curves. Myeloid antigen expression in adult ALL patients may occur more frequently than previously reported. The presence of myeloid antigen does not identify, in our series, a higher-risk subgroup of patients, although lack of any statistical evidence of prognostic significance needs to be confirmed in a larger case study.

KW - Acute lymphoblastic leukaemia

KW - Immunofluorescence

KW - Myeloid antigen markers

UR - http://www.scopus.com/inward/record.url?scp=0028319018&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028319018&partnerID=8YFLogxK

M3 - Article

C2 - 7947269

AN - SCOPUS:0028319018

VL - 87

SP - 286

EP - 292

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 2

ER -