Increased expression of nuclear factor-κB in bronchial biopsies from smokers and patients with COPD

A. Di Stefano, G. Caramori, T. Oates, A. Capelli, M. Lusuardi, I. Gnemmi, F. Ioli, K. F. Chung, C. F. Donner, P. J. Barnes, I. M. Adcock

Research output: Contribution to journalArticlepeer-review

Abstract

The expression of nuclear factor (NF)-κB is an indicator of cellular activation and of inflammatory mediator production. The aim of the present study was to characterise the expression and localisation of p65, the major subunit of NF-κB, in the bronchial mucosa of patients with chronic obstructive pulmonary disease (COPD), and to examine the relationship between p65 expression and disease status. Bronchial biopsies were obtained from 14 smokers with COPD, 17 smokers with normal lung function and 12 nonsmokers with normal lung function. The number of p65 positive (+) cells was quantified by immunohistochemistry and the expression of p65 in bronchial biopsies from the three groups was examined by Western blotting (WB). Smokers with normal lung function and patients with COPD had increased numbers of p65+ cells in the epithelium and increased p65 nuclear expression. In COPD patients the number of epithelial p65+ cells correlated with the degree of airflow limitation. WB analysis showed an increase in p65 in smokers with normal lung function and COPD patients (p

Original languageEnglish
Pages (from-to)556-563
Number of pages8
JournalEuropean Respiratory Journal
Volume20
Issue number3
DOIs
Publication statusPublished - Sep 2002

Keywords

  • Airflow limitation
  • Airway
  • Inflammation
  • Nuclear factor-κB
  • T-lymphocytes

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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